ABLB2_PHYIT
ID ABLB2_PHYIT Reviewed; 100 AA.
AC D0P1A8;
DT 08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2009, sequence version 1.
DT 03-AUG-2022, entry version 36.
DE RecName: Full=RxLR effector protein Avrblb2 {ECO:0000303|PubMed:19794118};
DE AltName: Full=Avirulence protein Avrblb2 {ECO:0000303|PubMed:19794118};
DE Flags: Precursor;
GN Name=Avrblb2 {ECO:0000303|PubMed:19794118};
GN Synonyms=PexRD39 {ECO:0000303|PubMed:19794118},
GN PexRD40 {ECO:0000303|PubMed:19794118}; ORFNames=PITG_20300;
OS Phytophthora infestans (strain T30-4) (Potato late blight agent).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=403677;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=T30-4;
RX PubMed=19741609; DOI=10.1038/nature08358;
RG The Broad Institute Genome Sequencing Platform;
RA Haas B.J., Kamoun S., Zody M.C., Jiang R.H., Handsaker R.E., Cano L.M.,
RA Grabherr M., Kodira C.D., Raffaele S., Torto-Alalibo T., Bozkurt T.O.,
RA Ah-Fong A.M., Alvarado L., Anderson V.L., Armstrong M.R., Avrova A.,
RA Baxter L., Beynon J., Boevink P.C., Bollmann S.R., Bos J.I., Bulone V.,
RA Cai G., Cakir C., Carrington J.C., Chawner M., Conti L., Costanzo S.,
RA Ewan R., Fahlgren N., Fischbach M.A., Fugelstad J., Gilroy E.M., Gnerre S.,
RA Green P.J., Grenville-Briggs L.J., Griffith J., Grunwald N.J., Horn K.,
RA Horner N.R., Hu C.H., Huitema E., Jeong D.H., Jones A.M., Jones J.D.,
RA Jones R.W., Karlsson E.K., Kunjeti S.G., Lamour K., Liu Z., Ma L.,
RA Maclean D., Chibucos M.C., McDonald H., McWalters J., Meijer H.J.,
RA Morgan W., Morris P.F., Munro C.A., O'Neill K., Ospina-Giraldo M.,
RA Pinzon A., Pritchard L., Ramsahoye B., Ren Q., Restrepo S., Roy S.,
RA Sadanandom A., Savidor A., Schornack S., Schwartz D.C., Schumann U.D.,
RA Schwessinger B., Seyer L., Sharpe T., Silvar C., Song J., Studholme D.J.,
RA Sykes S., Thines M., van de Vondervoort P.J., Phuntumart V., Wawra S.,
RA Weide R., Win J., Young C., Zhou S., Fry W., Meyers B.C., van West P.,
RA Ristaino J., Govers F., Birch P.R., Whisson S.C., Judelson H.S.,
RA Nusbaum C.;
RT "Genome sequence and analysis of the Irish potato famine pathogen
RT Phytophthora infestans.";
RL Nature 461:393-398(2009).
RN [2]
RP INDUCTION, FUNCTION, DOMAIN, AND MUTAGENESIS OF 43-ARG--ARG-46.
RX PubMed=19794118; DOI=10.1105/tpc.109.068247;
RA Oh S.K., Young C., Lee M., Oliva R., Bozkurt T.O., Cano L.M., Win J.,
RA Bos J.I., Liu H.Y., van Damme M., Morgan W., Choi D., Van der Vossen E.A.,
RA Vleeshouwers V.G., Kamoun S.;
RT "In planta expression screens of Phytophthora infestans RXLR effectors
RT reveal diverse phenotypes, including activation of the Solanum
RT bulbocastanum disease resistance protein Rpi-blb2.";
RL Plant Cell 21:2928-2947(2009).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH HOST C14 PROTEASE, AND
RP MUTAGENESIS OF 93-ALA--SER-100.
RX PubMed=22143776; DOI=10.1073/pnas.1112708109;
RA Bozkurt T.O., Schornack S., Win J., Shindo T., Ilyas M., Oliva R.,
RA Cano L.M., Jones A.M., Huitema E., van der Hoorn R.A., Kamoun S.;
RT "Phytophthora infestans effector AVRblb2 prevents secretion of a plant
RT immune protease at the haustorial interface.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:20832-20837(2011).
RN [4]
RP INDUCTION.
RX PubMed=28228125; DOI=10.1186/s12864-017-3585-x;
RA Ah-Fong A.M., Kim K.S., Judelson H.S.;
RT "RNA-seq of life stages of the oomycete Phytophthora infestans reveals
RT dynamic changes in metabolic, signal transduction, and pathogenesis genes
RT and a major role for calcium signaling in development.";
RL BMC Genomics 18:198-198(2017).
RN [5]
RP INDUCTION.
RX PubMed=29312401; DOI=10.3389/fpls.2017.02155;
RA Yin J., Gu B., Huang G., Tian Y., Quan J., Lindqvist-Kreuze H., Shan W.;
RT "Conserved RXLR effector genes of Phytophthora infestans expressed at the
RT early stage of potato infection are suppressive to host defense.";
RL Front. Plant Sci. 8:2155-2155(2017).
RN [6]
RP INTERACTION WITH HOST CALMODULIN, SUBCELLULAR LOCATION, FUNCTION, DOMAIN,
RP AND MUTAGENESIS OF 78-ARG--LYS-82.
RX PubMed=30984224; DOI=10.3389/fpls.2019.00374;
RA Naveed Z.A., Bibi S., Ali G.S.;
RT "The Phytophthora RXLR Effector Avrblb2 Modulates Plant Immunity by
RT Interfering With Ca2+ Signaling Pathway.";
RL Front. Plant Sci. 10:374-374(2019).
RN [7]
RP SUBCELLULAR LOCATION, AND FUNCTION.
RX PubMed=30329083; DOI=10.1093/jxb/ery360;
RA Wang S., McLellan H., Bukharova T., He Q., Murphy F., Shi J., Sun S.,
RA van Weymers P., Ren Y., Thilliez G., Wang H., Chen X., Engelhardt S.,
RA Vleeshouwers V., Gilroy E.M., Whisson S.C., Hein I., Wang X., Tian Z.,
RA Birch P.R.J., Boevink P.C.;
RT "Phytophthora infestans RXLR effectors act in concert at diverse
RT subcellular locations to enhance host colonization.";
RL J. Exp. Bot. 70:343-356(2019).
CC -!- FUNCTION: Secreted effector that acts as an elicitor of hypersensitive
CC response (HR) specifically on plants carrying defense protein Rpi-blb2
CC (PubMed:19794118). Enhances P.infestans colonization of Nicotiana
CC benthamiana leaves (PubMed:30329083). Interacts with, and subsequently
CC prevents secretion into the apoplast of the host papain-like cysteine
CC protease C14, thus promoting virulence by interfering with the
CC execution of host defenses (PubMed:22143776). Associates with
CC calmodulin at the host plasma membrane to interfere with plant defense-
CC associated calcium signaling in hosts (PubMed:30984224).
CC {ECO:0000269|PubMed:19794118, ECO:0000269|PubMed:22143776,
CC ECO:0000269|PubMed:30329083, ECO:0000269|PubMed:30984224}.
CC -!- SUBUNIT: Interacts with the host papain-like cysteine protease C14
CC (PubMed:22143776). Interacts with the host calmodulin
CC (PubMed:30984224). {ECO:0000269|PubMed:22143776,
CC ECO:0000269|PubMed:30984224}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:22143776,
CC ECO:0000269|PubMed:30329083, ECO:0000269|PubMed:30984224}. Host cell
CC membrane {ECO:0000269|PubMed:22143776, ECO:0000269|PubMed:30329083,
CC ECO:0000269|PubMed:30984224}. Note=AVRblb2 localization is essential
CC for its virulence function but not for its avirulence activity.
CC {ECO:0000269|PubMed:22143776}.
CC -!- INDUCTION: Expression is induced during host plant infection.
CC {ECO:0000269|PubMed:28228125, ECO:0000269|PubMed:29312401}.
CC -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC phosphate (Probable). The motif is not required for perception by
CC defense protein Rpi-blb2 (PubMed:19794118).
CC {ECO:0000269|PubMed:19794118, ECO:0000305|PubMed:19794118}.
CC -!- DOMAIN: The 8 residues at the C-terminus (residues 93 to 100) are
CC required for the correct subcellular location at the cell periphery or
CC around haustoria, the ability to enhance P.infestans growth, the
CC association with C14 protease, and apoplastic C14 accumulation.
CC {ECO:0000269|PubMed:22143776}.
CC -!- DOMAIN: The amino acids 78-82 in the C-terminal region of are important
CC for binding to host calmodulin. {ECO:0000269|PubMed:30984224}.
CC -!- MISCELLANEOUS: The Avrblb2 protein of Phytophthora infestans is a
CC polymorphic member of the RXLR class of secreted effectors. Especially,
CC the polymorphic amino acid at position 69 is critical for activation of
CC Rpi-blb2 hypersensitivity. The Avrblb2 homologs that are recognized by
CC Rpi-blb2 have 'Val-69', 'Ala-69', or 'Ile-69', whereas the those that
CC are not recognized have 'Phe-69'. {ECO:0000269|PubMed:19794118}.
CC -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR EMBL; DS028242; EEY54134.1; -; Genomic_DNA.
DR RefSeq; XP_002895918.1; XM_002895872.1.
DR AlphaFoldDB; D0P1A8; -.
DR EnsemblProtists; PITG_20300T0; PITG_20300T0; PITG_20300.
DR GeneID; 9469957; -.
DR KEGG; pif:PITG_20300; -.
DR VEuPathDB; FungiDB:PITG_20300; -.
DR HOGENOM; CLU_158959_0_0_1; -.
DR InParanoid; D0P1A8; -.
DR OMA; IPNIKLQ; -.
DR OrthoDB; 1628005at2759; -.
DR Proteomes; UP000006643; Partially assembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Host cell membrane; Host membrane; Membrane; Reference proteome; Secreted;
KW Signal; Virulence.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..100
FT /note="RxLR effector protein Avrblb2"
FT /id="PRO_5003013144"
FT MOTIF 43..57
FT /note="RxLR-dEER"
FT /evidence="ECO:0000305|PubMed:19794118"
FT MOTIF 78..82
FT /note="Calmodulin-binding motif"
FT /evidence="ECO:0000269|PubMed:30984224"
FT MUTAGEN 43..46
FT /note="RSLR->ASAA: Does not affect the perception by
FT defense protein Rpi-blb2."
FT /evidence="ECO:0000269|PubMed:19794118"
FT MUTAGEN 78..82
FT /note="RPDIK->AAAAA: Impairs the interaction with host
FT calmodulin."
FT /evidence="ECO:0000269|PubMed:30984224"
FT MUTAGEN 93..100
FT /note="Missing: Impairs specific accumulation at the cell
FT periphery or around haustoria, loses ability to enhance
FT P.infestans growth, strongly decreases association with C14
FT protease, and fails to attenuate apoplastic C14
FT accumulation."
FT /evidence="ECO:0000269|PubMed:22143776"
SQ SEQUENCE 100 AA; 10821 MW; 6CD6AFAEB92F1BDF CRC64;
MRSFLYGVLA FAVLARSSAV AAFPIPDESR PLSKTSPDTV APRSLRIEAQ EVIQSGRGDG
YGGFWKNVAQ STNKIVKRPD IKIGKLIEAA KKAKAKMTKS
