ID   BIOY_RHOCB              Reviewed;         190 AA.
AC   D5ARG8;
DT   31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   15-JUN-2010, sequence version 1.
DT   25-MAY-2022, entry version 57.
DE   RecName: Full=Biotin transporter BioY;
DE   AltName: Full=Biotin ECF transporter S component BioY;
GN   Name=bioY; OrderedLocusNames=RCAP_rcc03249;
OS   Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Rhodobacteraceae; Rhodobacter.
OX   NCBI_TaxID=272942;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-309 / NBRC 16581 / SB1003;
RX   PubMed=20418398; DOI=10.1128/jb.00366-10;
RA   Strnad H., Lapidus A., Paces J., Ulbrich P., Vlcek C., Paces V.,
RA   Haselkorn R.;
RT   "Complete genome sequence of the photosynthetic purple nonsulfur bacterium
RT   Rhodobacter capsulatus SB 1003.";
RL   J. Bacteriol. 192:3545-3546(2010).
RN   [2]
RP   EXPRESSION IN E.COLI, FUNCTION IN BIOTIN UPTAKE, SUBCELLULAR LOCATION, AND
RP   SUBUNIT.
RC   STRAIN=ATCC BAA-309 / NBRC 16581 / SB1003;
RX   PubMed=17301237; DOI=10.1073/pnas.0609905104;
RA   Hebbeln P., Rodionov D.A., Alfandega A., Eitinger T.;
RT   "Biotin uptake in prokaryotes by solute transporters with an optional ATP-
RT   binding cassette-containing module.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:2909-2914(2007).
CC   -!- FUNCTION: Central protein that confers the ability to take up biotin on
CC       E.coli; while biotin transport requires BioM at very low (pM)
CC       concentrations at higher (nM) concentrations expression of BioY alone
CC       suffices for uptake. {ECO:0000269|PubMed:17301237}.
CC   -!- SUBUNIT: Part of a biotin transporter holocomplex composed of BioM,
CC       BioN and BioY. BioMN complexes can also be readily purified, but not
CC       BioMY nor BioNY complexes. BioY homodimers are also seen. Only the
CC       BioMNY complex has ATPase activity. {ECO:0000269|PubMed:17301237}.
CC   -!- INTERACTION:
CC       D5ARG8; D5ARG8: bioY; NbExp=3; IntAct=EBI-6418966, EBI-6418966;
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000305|PubMed:17301237}; Multi-pass membrane protein
CC       {ECO:0000305|PubMed:17301237}.
CC   -!- SIMILARITY: Belongs to the BioY family. {ECO:0000305}.
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DR   EMBL; CP001312; ADE86973.1; -; Genomic_DNA.
DR   RefSeq; WP_013068946.1; NC_014034.1.
DR   AlphaFoldDB; D5ARG8; -.
DR   SMR; D5ARG8; -.
DR   STRING; 272942.RCAP_rcc03249; -.
DR   TCDB; 2.A.88.1.3; the vitamin uptake transporter (vut) family.
DR   TCDB; 3.A.1.25.7; the atp-binding cassette (abc) superfamily.
DR   EnsemblBacteria; ADE86973; ADE86973; RCAP_rcc03249.
DR   GeneID; 31492030; -.
DR   KEGG; rcp:RCAP_rcc03249; -.
DR   eggNOG; COG1268; Bacteria.
DR   HOGENOM; CLU_077931_0_1_5; -.
DR   OMA; GMVINYV; -.
DR   OrthoDB; 1691691at2; -.
DR   Proteomes; UP000002361; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015225; F:biotin transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   InterPro; IPR003784; BioY.
DR   PANTHER; PTHR34295; PTHR34295; 1.
DR   Pfam; PF02632; BioY; 1.
DR   PIRSF; PIRSF016661; BioY; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..190
FT                   /note="Biotin transporter BioY"
FT                   /id="PRO_0000409006"
FT   TRANSMEM        8..28
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        29..49
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        51..71
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        76..96
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        115..135
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        146..166
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   190 AA;  19306 MW;  7C4EABBAE63D0E13 CRC64;
     MERNVTLIGL FAALIVALGF VPAIPLGFGV PITLQSLGVM LAGAVLGSWR GALAVLLVQA
     LVAIGLPVLA GGRGGLGIFV GPTAGFLIGW LPAAFVTGLI VERLRRVPVA LAAGIGATLG
     GIIIMYALGI LGFWLVKNAG LKPEDAPISL WAATAIMAPF IPGDLVKVVV TGLVARTIAQ
     YRPSALLARG