BIOY_THEMA
ID BIOY_THEMA Reviewed; 169 AA.
AC Q9WZQ6; G4FD25;
DT 01-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 25-MAY-2022, entry version 89.
DE RecName: Full=Biotin transporter BioY;
DE AltName: Full=Biotin ECF transporter S component BioY;
GN Name=bioY; OrderedLocusNames=TM_0799;
OS Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826
OS / MSB8).
OC Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga.
OX NCBI_TaxID=243274;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX PubMed=10360571; DOI=10.1038/20601;
RA Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H.,
RA Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A.,
RA Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M.,
RA Cotton M.D., Pratt M.S., Phillips C.A., Richardson D.L., Heidelberg J.F.,
RA Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L.,
RA Smith H.O., Venter J.C., Fraser C.M.;
RT "Evidence for lateral gene transfer between Archaea and Bacteria from
RT genome sequence of Thermotoga maritima.";
RL Nature 399:323-329(1999).
RN [2]
RP PROBABLE FUNCTION AS A TRANSPORT COMPONENT, SUBUNIT, SUBCELLULAR LOCATION,
RP AND EXPRESSION IN E.COLI.
RC STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX PubMed=23359690; DOI=10.1073/pnas.1217361110;
RA Karpowich N.K., Wang D.N.;
RT "Assembly and mechanism of a group II ECF transporter.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:2534-2539(2013).
CC -!- FUNCTION: Substrate-binding (S) component of an energy-coupling factor
CC (ECF) ABC-transporter complex. Probably a biotin-binding protein that
CC interacts with the energy-coupling factor (ECF) ABC-transporter
CC complex. Unlike classic ABC transporters this ECF transporter provides
CC the energy necessary to transport a number of different substrates. The
CC substrates themselves are bound by transmembrane, not extracytoplasmic
CC soluble proteins (Probable). {ECO:0000305}.
CC -!- SUBUNIT: Forms a stable energy-coupling factor (ECF) transporter
CC complex composed of 2 membrane-embedded substrate-binding proteins (S
CC component, RibU, BioY), 2 ATP-binding proteins (A component) and 2
CC transmembrane proteins (T component) upon coexpression in E.coli. A
CC stable subcomplex with both A and T components are also isolated. This
CC complex interacts with at least 2 substrate-specific components, BioY
CC and RibU. {ECO:0000269|PubMed:23359690}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000305|PubMed:23359690}; Multi-pass membrane protein
CC {ECO:0000305|PubMed:23359690}.
CC -!- SIMILARITY: Belongs to the BioY family. {ECO:0000305}.
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DR EMBL; AE000512; AAD35881.1; -; Genomic_DNA.
DR PIR; H72334; H72334.
DR RefSeq; NP_228608.1; NC_000853.1.
DR RefSeq; WP_004080866.1; NZ_CP011107.1.
DR AlphaFoldDB; Q9WZQ6; -.
DR SMR; Q9WZQ6; -.
DR STRING; 243274.THEMA_00655; -.
DR TCDB; 3.A.1.25.5; the atp-binding cassette (abc) superfamily.
DR EnsemblBacteria; AAD35881; AAD35881; TM_0799.
DR KEGG; tma:TM0799; -.
DR eggNOG; COG1268; Bacteria.
DR InParanoid; Q9WZQ6; -.
DR OMA; AGYLWSY; -.
DR OrthoDB; 1691691at2; -.
DR Proteomes; UP000008183; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015225; F:biotin transmembrane transporter activity; IEA:InterPro.
DR InterPro; IPR003784; BioY.
DR PANTHER; PTHR34295; PTHR34295; 1.
DR Pfam; PF02632; BioY; 1.
DR PIRSF; PIRSF016661; BioY; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..169
FT /note="Biotin transporter BioY"
FT /id="PRO_0000422263"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 47..67
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 75..95
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 106..126
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..159
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 169 AA; 18235 MW; C99E94F178266729 CRC64;
MRQLIKAGIF TALIVVGAWI SIPLGPVPFT LQVFFVFLSA YVLGKKYGTL AVATYVLLGA
MGLPVFANFK GGAQVLVGPT GGYLFGFILG AFVIGLLAEK KESFAWYLAS GVAGLGIIYA
LGVFVLNFYV HDIRKAISVG FVPFVWFDLI KLVVAALIAL RLKKLEVER
