SYF1_DROME
ID SYF1_DROME Reviewed; 883 AA.
AC A1Z9G2; C3KGI3;
DT 18-SEP-2019, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Pre-mRNA-splicing factor syf1 homolog {ECO:0000250|UniProtKB:Q9HCS7};
DE AltName: Full=Pre-mRNA-splicing factor fandango {ECO:0000303|PubMed:24755291};
DE AltName: Full=Protein faint sausage {ECO:0000303|PubMed:28087625};
GN Name=fand {ECO:0000303|PubMed:24755291, ECO:0000312|FlyBase:FBgn0033859};
GN Synonyms=fas {ECO:0000303|PubMed:28087625};
GN ORFNames=CG6197 {ECO:0000312|FlyBase:FBgn0033859};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:ACO95726.1, ECO:0000312|EMBL:AEZ68806.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:ACO95726.1, ECO:0000312|EMBL:AEZ68806.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:ACO95726.1};
RA Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.;
RL Submitted (FEB-2012) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305}
RP FUNCTION, AND MUTAGENESIS OF 69-TRP--GLU-883.
RX PubMed=10502111; DOI=10.1007/s004270050287;
RA Jack J., Myette G.;
RT "Mutations that alter the morphology of the malpighian tubules in
RT Drosophila.";
RL Dev. Genes Evol. 209:546-554(1999).
RN [5] {ECO:0000305}
RP FUNCTION, INTERACTION WITH CG9667 AND PRP19, SUBCELLULAR LOCATION,
RP DEVELOPMENTAL STAGE, AND MUTAGENESIS OF 355-ASN--HIS-360 AND ALA-401.
RX PubMed=24755291; DOI=10.7554/elife.02181;
RA Guilgur L.G., Prudencio P., Sobral D., Liszekova D., Rosa A.,
RA Martinho R.G.;
RT "Requirement for highly efficient pre-mRNA splicing during Drosophila early
RT embryonic development.";
RL Elife 3:E02181-E02181(2014).
RN [6] {ECO:0000305}
RP FUNCTION, AND MUTAGENESIS OF 69-TRP--GLU-883 AND 219-TRP--GLU-883.
RX PubMed=28087625; DOI=10.1242/dev.144535;
RA Sauerwald J., Soneson C., Robinson M.D., Luschnig S.;
RT "Faithful mRNA splicing depends on the Prp19 complex subunit faint sausage
RT and is required for tracheal branching morphogenesis in Drosophila.";
RL Development 144:657-663(2017).
CC -!- FUNCTION: Subunit of the NTC(Nineteen)/Prp19 complex, which is part of
CC the spliceosome (PubMed:24755291, PubMed:28087625). The complex
CC participates in spliceosome assembly, its remodeling and is required
CC for efficient spliceosome activation (PubMed:24755291,
CC PubMed:28087625). Essential for efficient pre-mRNA splicing
CC (PubMed:24755291, PubMed:28087625). In embryos, efficient pre-mRNA
CC splicing of zygotic transcripts is essential during dynamic cellular
CC processes that require rapid division and/or dramatic changes in gene
CC expression such as blastoderm cellularization, tracheal branching
CC morphogenesis, Malpighian morphogenesis and epidermal development
CC (PubMed:24755291, PubMed:28087625, PubMed:10502111). Part of its role
CC in promoting embryo tracheal development is also due to specifically
CC splicing bnl transcripts which results in the activation of the BNL-FGF
CC pathway (PubMed:28087625). {ECO:0000269|PubMed:10502111,
CC ECO:0000269|PubMed:24755291, ECO:0000269|PubMed:28087625}.
CC -!- SUBUNIT: Component of the NTC(Nineteen)/Prp19 complex composed of at
CC least fand, Prp19,CG9667/ISY1 and Cdc5/CDC5L. Within the complex,
CC interacts with Prp19 and ISY1/CG9667. {ECO:0000269|PubMed:24755291}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:24755291}.
CC -!- DEVELOPMENTAL STAGE: Expressed in adult ovaries and 0-3 hours embryos
CC (at protein level). {ECO:0000269|PubMed:24755291}.
CC -!- MISCELLANEOUS: Named 'fandango' after the Iberian folk dance to
CC highlight the similarity of the blastoderm cellularization phenotype to
CC the charleston/kuk phenotype (PubMed:24755291). The phenotype 'faint
CC sausage (fas)', consisting of a poorly differentiated cuticle and head,
CC was previously associated with the immunoglobulin domain protein
CC CG17716 but is now known to be caused by mutations in the protein
CC 'fandango' (PubMed:24755291). {ECO:0000269|PubMed:24755291,
CC ECO:0000303|PubMed:24755291}.
CC -!- SIMILARITY: Belongs to the crooked-neck family. {ECO:0000305}.
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DR EMBL; AE013599; AAF58348.1; -; Genomic_DNA.
DR EMBL; BT082048; ACO95726.1; -; mRNA.
DR EMBL; BT133178; AEZ68806.1; -; mRNA.
DR RefSeq; NP_610891.1; NM_137047.3.
DR AlphaFoldDB; A1Z9G2; -.
DR SMR; A1Z9G2; -.
DR IntAct; A1Z9G2; 5.
DR STRING; 7227.FBpp0086749; -.
DR PaxDb; A1Z9G2; -.
DR PRIDE; A1Z9G2; -.
DR DNASU; 36514; -.
DR EnsemblMetazoa; FBtr0087623; FBpp0086749; FBgn0033859.
DR GeneID; 36514; -.
DR KEGG; dme:Dmel_CG6197; -.
DR UCSC; CG6197-RA; d. melanogaster.
DR CTD; 36514; -.
DR FlyBase; FBgn0033859; fand.
DR VEuPathDB; VectorBase:FBgn0033859; -.
DR eggNOG; KOG2047; Eukaryota.
DR GeneTree; ENSGT00550000075140; -.
DR HOGENOM; CLU_007736_0_0_1; -.
DR InParanoid; A1Z9G2; -.
DR OMA; FLMQQPL; -.
DR OrthoDB; 370051at2759; -.
DR PhylomeDB; A1Z9G2; -.
DR Reactome; R-DME-6781823; Formation of TC-NER Pre-Incision Complex.
DR Reactome; R-DME-6782135; Dual incision in TC-NER.
DR Reactome; R-DME-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR Reactome; R-DME-72163; mRNA Splicing - Major Pathway.
DR SignaLink; A1Z9G2; -.
DR BioGRID-ORCS; 36514; 1 hit in 1 CRISPR screen.
DR ChiTaRS; fas; fly.
DR GenomeRNAi; 36514; -.
DR PRO; PR:A1Z9G2; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0033859; Expressed in embryonic/larval hemocyte (Drosophila) and 21 other tissues.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; HDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IC:FlyBase.
DR GO; GO:0071014; C:post-mRNA release spliceosomal complex; IBA:GO_Central.
DR GO; GO:0071011; C:precatalytic spliceosome; HDA:FlyBase.
DR GO; GO:0000974; C:Prp19 complex; IDA:FlyBase.
DR GO; GO:0071007; C:U2-type catalytic step 2 spliceosome; IBA:GO_Central.
DR GO; GO:0007510; P:cardioblast cell fate determination; IMP:FlyBase.
DR GO; GO:0000349; P:generation of catalytic spliceosome for first transesterification step; IBA:GO_Central.
DR GO; GO:0007443; P:Malpighian tubule morphogenesis; IMP:FlyBase.
DR GO; GO:0002009; P:morphogenesis of an epithelium; IMP:FlyBase.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; HMP:FlyBase.
DR GO; GO:0008380; P:RNA splicing; IMP:FlyBase.
DR Gene3D; 1.25.40.10; -; 3.
DR InterPro; IPR003107; HAT.
DR InterPro; IPR045075; Syf1-like.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PANTHER; PTHR11246; PTHR11246; 1.
DR SMART; SM00386; HAT; 13.
DR SUPFAM; SSF48452; SSF48452; 5.
PE 1: Evidence at protein level;
KW mRNA processing; mRNA splicing; Nucleus; Reference proteome; Repeat;
KW Spliceosome.
FT CHAIN 1..883
FT /note="Pre-mRNA-splicing factor syf1 homolog"
FT /id="PRO_0000447890"
FT REPEAT 13..45
FT /note="HAT 1"
FT /evidence="ECO:0000255"
FT REPEAT 46..78
FT /note="HAT 2"
FT /evidence="ECO:0000255"
FT REPEAT 88..120
FT /note="HAT 3"
FT /evidence="ECO:0000255"
FT REPEAT 122..156
FT /note="HAT 4"
FT /evidence="ECO:0000255"
FT REPEAT 158..190
FT /note="HAT 5"
FT /evidence="ECO:0000255"
FT REPEAT 268..303
FT /note="HAT 6"
FT /evidence="ECO:0000255"
FT REPEAT 368..406
FT /note="HAT 7"
FT /evidence="ECO:0000255"
FT REPEAT 463..495
FT /note="HAT 8"
FT /evidence="ECO:0000255"
FT REPEAT 531..565
FT /note="HAT 9"
FT /evidence="ECO:0000255"
FT REPEAT 570..604
FT /note="HAT 10"
FT /evidence="ECO:0000255"
FT REPEAT 642..676
FT /note="HAT 11"
FT /evidence="ECO:0000255"
FT REPEAT 678..712
FT /note="HAT 12"
FT /evidence="ECO:0000255"
FT REGION 794..851
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 864..883
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 798..812
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 813..835
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 69..883
FT /note="Missing: Embryonic lethal and displays defects in
FT primary tracheal branching and Malpighian morphogenesis.
FT Displays abnormal splicing of zygotic transcripts resulting
FT in qualitative (intron retention) as well as quantitative
FT (transcript abundance) changes in gene expression. Tracheal
FT defects are likely due, at least in part, to the mis-
FT splicing of bnl transcripts. Embryonic Malpighian tubules
FT are shortened and round."
FT /evidence="ECO:0000269|PubMed:10502111,
FT ECO:0000269|PubMed:28087625"
FT MUTAGEN 219..883
FT /note="Missing: H124; embryonic lethal and displays defects
FT in primary tracheal branching. Tracheal defects are likely
FT due at least in part, to the mis-splicing of bnl
FT transcripts. Earlier developmental processes, such as
FT gastrulation and germband retraction are unaffected."
FT /evidence="ECO:0000269|PubMed:28087625"
FT MUTAGEN 355..360
FT /note="Missing: Fand2; embryos display significant splicing
FT defects in the small zygotic kuk transcript whereas there
FT is no effect on splicing of the large maternal kuk
FT transcript. Reduced protein levels of ISY1/CG9667 but no
FT effect on Prp19."
FT /evidence="ECO:0000269|PubMed:24755291"
FT MUTAGEN 401
FT /note="A->V: Fand1; a large subset of zygotic but not
FT maternally encoded pre-mRNAs, including the kuk transcript,
FT display splicing defects. Consequently, maternally
FT controlled oogenesis, such as primordial germ-cell
FT formation and syncytial nuclear divisions, appear normal
FT but during zygotically controlled blastoderm
FT cellularization the cortical nuclei fail to elongate and
FT become mislocalized. Reduced protein levels of ISY1/CG9667
FT but no effect on Prp19."
FT /evidence="ECO:0000269|PubMed:24755291"
FT CONFLICT 575
FT /note="A -> V (in Ref. 3; ACO95726)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 883 AA; 103327 MW; 35F5D93C849954FF CRC64;
MVTKTIKSLN LEINFEVEDV PYEEEILRNA YSVKHWLRYI DHKAKAPNNG VNMVYERALK
ELPGSYKIWH NYLRTRRKQV RGKIPTDPMY EEVNSAFERA LVFMHKMPRI WMDYGAFMTS
QCKITRTRHV FDRALRALPI TQHGRIWPLY LQFVRRFEMP ETALRVYRRY LKLFPEDTEE
YVDYLQEADR LDEAAQQLAH IVDNEHFVSK HGKSNHQLWN ELCDLISKNP HKVHSLNVDA
IIRGGLRRYT DQLGHLWNSL ADYYVRSGLF DRARDIYEEA IQTVTTVRDF TQVFDEYAQF
EELSLNRRME QVAANEAATE EDDIDVELRL SRFEYLMERR LLLLNSVLLR QNPHNVHEWH
KRVTLYEDKP AEIISTYTEA VQTVQPKQAV GKLHTLWVEF AKFYEANGQV EDARVVFERG
TEVEYVKVED LAAVWCEWAE MELRQQQFEA ALKLMQRATA MPKRKIAYYD DTETVQARLH
RSLKVWSMYA DLEESFGTFK TCKAVYERII DLKICTPQII INYGMFLEEH NYFEEAYRAY
EKGISLFKWP NVYDIWNSYL TKFLERYGGT KLERARDLFE QCLDQCPPEH AKYFYLLYAK
LEEEHGLARH AMSVYDRATS AVKEDEMFDM YNIFIKKAAE IYGLPRTREI YEKAIESLPE
QNMRHMCVKF AELETKLGEV DRARAIYAHC SQVCDPRITA DFWQTWKEFE VRHGNEDTMR
EMLRIKRSVQ ATYNTQVNMM AAQFLSTNNG AAADAGAGAG PDAMRLLEEK ARQAAAESKQ
KPIEKAASNI MFVRGETQGG AKDKKDTVVN PDEIDIGDSD EDDEEEDDDE ENEMTNENQA
SAAVTKTDEE GLVMKKLRFE QKAIPAKVFG SLKPSNQGDS DGE
