BIR1B_MOUSE
ID BIR1B_MOUSE Reviewed; 1447 AA.
AC Q9QUK4; A9C470; O09124; Q9R030;
DT 24-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=Baculoviral IAP repeat-containing protein 1b;
DE AltName: Full=Neuronal apoptosis inhibitory protein 2;
GN Name=Naip2; Synonyms=Birc1b, Naip-rs6;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=10501978; DOI=10.1007/s003359901155;
RA Huang S., Scharf J.M., Growney J.D., Endrizzi M.G., Dietrich W.F.;
RT "The mouse Naip gene cluster on chromosome 13 encodes several distinct
RT functional transcripts.";
RL Mamm. Genome 10:1032-1035(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=10384056; DOI=10.1007/s003359901087;
RA Yaraghi Z., Diez E., Gros P., MacKenzie A.;
RT "cDNA cloning and the 5'genomic organization of Naip2, a candidate gene for
RT murine Legionella resistance.";
RL Mamm. Genome 10:761-763(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/Sv;
RX PubMed=10486205; DOI=10.1006/geno.1999.5910;
RA Endrizzi M., Huang S., Scharf J.M., Kelter A.R., Wirth B., Kunkel L.M.,
RA Miller W., Dietrich W.F.;
RT "Comparative sequence analysis of the mouse and human Lgn1/SMA interval.";
RL Genomics 60:137-151(1999).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 82-168.
RC STRAIN=129/SvJ;
RX PubMed=8975718; DOI=10.1006/geno.1996.0644;
RA Scharf J.M., Damron D., Frisella A., Bruno S., Beggs A.H., Kunkel L.M.,
RA Dietrich W.F.;
RT "The mouse region syntenic for human spinal muscular atrophy lies within
RT the Lgn1 critical interval and contains multiple copies of Naip exon 5.";
RL Genomics 38:405-417(1996).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [7]
RP FUNCTION, AND INTERACTION WITH S.TYPHIMURIUM PRGJ.
RX PubMed=21874021; DOI=10.1038/nature10394;
RA Kofoed E.M., Vance R.E.;
RT "Innate immune recognition of bacterial ligands by NAIPs determines
RT inflammasome specificity.";
RL Nature 477:592-595(2011).
RN [8]
RP FUNCTION, AND INTERACTION WITH S.TYPHIMURIUM PRGJ AND B.THAILANDENSIS BSAK.
RX PubMed=21918512; DOI=10.1038/nature10510;
RA Zhao Y., Yang J., Shi J., Gong Y.N., Lu Q., Xu H., Liu L., Shao F.;
RT "The NLRC4 inflammasome receptors for bacterial flagellin and type III
RT secretion apparatus.";
RL Nature 477:596-600(2011).
CC -!- FUNCTION: Sensor component of the NLRC4 inflammasome that specifically
CC recognizes and binds type III secretion system (T3SS) rod proteins such
CC as S.typhimurium (Salmonella) PrgJ and B.thailandensis BsaK from
CC pathogenic bacteria. Association of pathogenic bacteria proteins drives
CC in turn drive assembly and activation of the NLRC4 inflammasome,
CC promoting caspase-1 activation, cytokine production and macrophage
CC pyroptosis. The NLRC4 inflammasome is activated as part of the innate
CC immune response to a range of intracellular bacteria. The NLRC4
CC inflammasome senses Gram-negative bacteria such as L.pneumophila and
CC P.aeruginosa, enteric pathogens S.typhimurium (Salmonella) and
CC S.flexneri. Prevents motor-neuron apoptosis induced by a variety of
CC signals. {ECO:0000269|PubMed:21874021, ECO:0000269|PubMed:21918512}.
CC -!- SUBUNIT: Component of the NLRC4 inflammasome, at least composed of
CC NLRC4, caspase-1 (CASP1) and some NAIP protein. Interacts with
CC S.typhimurium (Salmonella) PrgJ and B.thailandensis BsaK.
CC {ECO:0000269|PubMed:21874021, ECO:0000269|PubMed:21918512}.
CC -!- INTERACTION:
CC Q9QUK4; Q2T728: BTH_II0824; Xeno; NbExp=2; IntAct=EBI-15944068, EBI-15944060;
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DR EMBL; AF135489; AAD56761.1; -; mRNA.
DR EMBL; AF135490; AAD56762.1; -; mRNA.
DR EMBL; AF102871; AAC73002.1; -; mRNA.
DR EMBL; AF131205; AAD56759.1; -; Genomic_DNA.
DR EMBL; CT030167; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; U66329; AAC52977.1; -; Genomic_DNA.
DR CCDS; CCDS26726.1; -.
DR PIR; T42628; T42628.
DR RefSeq; NP_001119654.1; NM_001126182.2.
DR RefSeq; NP_035002.2; NM_010872.3.
DR AlphaFoldDB; Q9QUK4; -.
DR SMR; Q9QUK4; -.
DR DIP; DIP-59160N; -.
DR IntAct; Q9QUK4; 4.
DR STRING; 10090.ENSMUSP00000113890; -.
DR MEROPS; I32.001; -.
DR iPTMnet; Q9QUK4; -.
DR PhosphoSitePlus; Q9QUK4; -.
DR MaxQB; Q9QUK4; -.
DR PaxDb; Q9QUK4; -.
DR PRIDE; Q9QUK4; -.
DR ProteomicsDB; 273616; -.
DR DNASU; 17948; -.
DR Ensembl; ENSMUST00000067975; ENSMUSP00000070827; ENSMUSG00000078945.
DR Ensembl; ENSMUST00000117913; ENSMUSP00000113890; ENSMUSG00000078945.
DR GeneID; 17948; -.
DR KEGG; mmu:17948; -.
DR UCSC; uc007rqj.2; mouse.
DR CTD; 17948; -.
DR MGI; MGI:1298226; Naip2.
DR VEuPathDB; HostDB:ENSMUSG00000078945; -.
DR eggNOG; KOG1101; Eukaryota.
DR GeneTree; ENSGT00940000163369; -.
DR InParanoid; Q9QUK4; -.
DR OMA; FENWPFY; -.
DR OrthoDB; 268914at2759; -.
DR PhylomeDB; Q9QUK4; -.
DR TreeFam; TF105356; -.
DR BioGRID-ORCS; 17948; 3 hits in 71 CRISPR screens.
DR PRO; PR:Q9QUK4; -.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; Q9QUK4; protein.
DR Bgee; ENSMUSG00000078945; Expressed in granulocyte and 98 other tissues.
DR ExpressionAtlas; Q9QUK4; baseline and differential.
DR Genevisible; Q9QUK4; MM.
DR GO; GO:0072557; C:IPAF inflammasome complex; IDA:UniProtKB.
DR GO; GO:0005524; F:ATP binding; ISS:UniProtKB.
DR GO; GO:0043027; F:cysteine-type endopeptidase inhibitor activity involved in apoptotic process; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0120283; F:protein serine/threonine kinase binding; ISO:MGI.
DR GO; GO:0071391; P:cellular response to estrogen stimulus; IDA:MGI.
DR GO; GO:0042742; P:defense response to bacterium; IMP:UniProtKB.
DR GO; GO:0016045; P:detection of bacterium; IMP:UniProtKB.
DR GO; GO:0006954; P:inflammatory response; IMP:UniProtKB.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:0043154; P:negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; ISO:MGI.
DR GO; GO:0010804; P:negative regulation of tumor necrosis factor-mediated signaling pathway; ISO:MGI.
DR GO; GO:0046330; P:positive regulation of JNK cascade; ISO:MGI.
DR GO; GO:0070269; P:pyroptosis; IMP:UniProtKB.
DR GO; GO:0042981; P:regulation of apoptotic process; ISO:MGI.
DR CDD; cd00022; BIR; 3.
DR Gene3D; 3.40.50.300; -; 1.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR001370; BIR_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR007111; NACHT_NTPase.
DR InterPro; IPR028789; Naip.
DR InterPro; IPR040535; NLRC4_HD.
DR InterPro; IPR041075; NOD2_WH.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR46914; PTHR46914; 1.
DR Pfam; PF00653; BIR; 3.
DR Pfam; PF05729; NACHT; 1.
DR Pfam; PF17889; NLRC4_HD; 1.
DR Pfam; PF17779; NOD2_WH; 1.
DR SMART; SM00238; BIR; 3.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS01282; BIR_REPEAT_1; 2.
DR PROSITE; PS50143; BIR_REPEAT_2; 3.
DR PROSITE; PS50837; NACHT; 1.
PE 1: Evidence at protein level;
KW Apoptosis; ATP-binding; Immunity; Inflammatory response; Innate immunity;
KW Metal-binding; Nucleotide-binding; Reference proteome; Repeat; Zinc.
FT CHAIN 1..1447
FT /note="Baculoviral IAP repeat-containing protein 1b"
FT /id="PRO_0000122343"
FT REPEAT 60..127
FT /note="BIR 1"
FT REPEAT 159..227
FT /note="BIR 2"
FT REPEAT 278..345
FT /note="BIR 3"
FT DOMAIN 508..802
FT /note="NACHT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00136"
FT BINDING 315
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00029"
FT BINDING 318
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00029"
FT BINDING 335
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00029"
FT BINDING 342
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00029"
FT BINDING 520
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT CONFLICT 377
FT /note="D -> G (in Ref. 3; AAD56759)"
FT /evidence="ECO:0000305"
FT CONFLICT 403
FT /note="L -> F (in Ref. 3; AAD56759)"
FT /evidence="ECO:0000305"
FT CONFLICT 478
FT /note="L -> I (in Ref. 3; AAD56759)"
FT /evidence="ECO:0000305"
FT CONFLICT 540
FT /note="Y -> N (in Ref. 1; AAD56761/AAD56762 and 2;
FT AAC73002)"
FT /evidence="ECO:0000305"
FT CONFLICT 862
FT /note="K -> N (in Ref. 3; AAD56759)"
FT /evidence="ECO:0000305"
FT CONFLICT 1079..1080
FT /note="SD -> FN (in Ref. 3; AAD56759)"
FT /evidence="ECO:0000305"
FT CONFLICT 1089
FT /note="R -> C (in Ref. 3; AAD56759)"
FT /evidence="ECO:0000305"
FT CONFLICT 1115
FT /note="K -> E (in Ref. 3; AAD56759)"
FT /evidence="ECO:0000305"
FT CONFLICT 1122
FT /note="T -> A (in Ref. 3; AAD56759)"
FT /evidence="ECO:0000305"
FT CONFLICT 1136
FT /note="D -> E (in Ref. 3; AAD56759)"
FT /evidence="ECO:0000305"
FT CONFLICT 1157
FT /note="S -> G (in Ref. 3; AAD56759)"
FT /evidence="ECO:0000305"
FT CONFLICT 1167
FT /note="G -> R (in Ref. 3; AAD56759)"
FT /evidence="ECO:0000305"
FT CONFLICT 1271
FT /note="F -> C (in Ref. 3; AAD56759)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1447 AA; 164083 MW; 9A9DFDA40B866393 CRC64;
MAAQGEAVEE IICEFDDDLV SELSTLLRVD ALSVLKRQQE EDHKTRMKMK KGFNSQMRSE
AKRLKTFETY DKFRSWTPQE MAAAGFYHTG VKLGVQCFCC SLILFSTRLR KLPIENHKKL
RPECEFLLGK DVGNIGKYDI RVKSPEKMLR GDKARYHEEE ARLESFEDWP FYAHGTSPRV
LSAAGFVFTG KRDTVQCFSC GGCLGNWEEG DDPWKEHAKW FPKCEFLQSK KSPEEITQYV
QSYEGFLHVT GEHFVNSWVR RELPMVSAYC NDSVFANEEL RMDTFKDWPH ESPGAVEALV
KAGLFYTGKR DIVQCFSCGG CMEKWAEGDN PIEDHTKFFP NCVFLQTLKS SAEVIPALQS
HCALPEAMET TSESNHDDAA AVHSTVVDVS PSEAQELEPA SSLVSVLCRD QDHSEAQGRG
CASSGTYLPS TDLGQSEAQW LQEARSLSEQ LRDTYTKATF RHMNLPEVYS SLGTDHLLSC
DVSIISKHIS QPVQGSLTIP EVFSNLNSVM CVEGEAGSGK TTFLKRIAFL WASGCCPLLY
RFQLVFYLSL SSITPGQELA KIICAQLLGA GGCISEVCLS SIIQQLQHQV LFLLDDYSGL
ASLPQALHTL ITKNYLSRTC LLIAVHTNKV RGIRPYLDTS LEIKEFPFYN TVSVLRKLFS
HDIMRVRKFI NYFGFHEELQ GIHKTPLFVA AVCTDWFKNP SDQPFQDVAL FKAYMQYLSL
KHKGAAKPLQ ATVSSCGQLA LTGLFSSCFE FNSDNLAEAG VDEDEELTTC LMSKFTAQRL
RPVYRFLGPL FQEFLAAVRL TELLSSDRQE DQDLGLYYLR QINSPLKAMS IYHTFLKYVS
SHPSSKAAPT VVSHLLQLVD EKESLENMSE NEDYMKLHPE ALLWIECLRG LWQLSPESFS
LFISENLLRI CLNFAHESNT VAACSPVILQ FLRGRTLDLK VLSLQYFWDH PETLLLLKSI
KISLNGNNWV QRIDFSLIEK SFEKVQPPTI DQDYAIAFQP INEVQKNLSE KKHIIKKYED
MKHQIPLNIS TGYWKLSPKP YKIPKLEVQV TNTGPADQAL LQVLMEVFSA SQSIEFRLSD
SSGFLESIRP ALELSKASVT KCSMSRLELS REDQKLLLTL PTLQSLEVSE TNQLPDQLFH
NLHKFLGLKE LCVRLDSKPD VLSVLPGEFP NLHHMEKLSI RTSTESDLSK LVKLIQNSPN
LHVFHLKCNF LSNCEPLMTV LASCKKLREI EFSGRCFEAM TFVNILPNFV FLKILNLRDQ
QFPDKETSEK FAQALGSLRN LEKLFVPTGD GIHQVAKLIV RQCLQLPCLR VLVFAETLDD
DSVLEIAKGA TRGGFQKLEN LDLTLNHKIT EEGYRNFFQV LDNLPNLKNL DISRHIPECI
QIQAITVKAL GQCVSRLPSL TRLGMLSWLL DEEDIKVIND VKERHPQSKR LTVHWRWVVP
FSPVIQK
