ID   ABM_MAGO7               Reviewed;         215 AA.
AC   P0CU79;
DT   16-JAN-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-JAN-2019, sequence version 1.
DT   25-MAY-2022, entry version 7.
DE   RecName: Full=Jasmonate monooxygenase ABM {ECO:0000303|PubMed:26258762};
DE            EC=1.14.13.228 {ECO:0000269|PubMed:26258762};
DE   AltName: Full=Antibiotic biosynthesis monooxygenase {ECO:0000303|PubMed:26258762};
DE            Short=ABM {ECO:0000303|PubMed:26258762};
GN   Name=ABM {ECO:0000303|PubMed:26258762}; ORFNames=MGG_04777;
OS   Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast
OS   fungus) (Pyricularia oryzae).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Magnaporthales; Pyriculariaceae; Pyricularia.
OX   NCBI_TaxID=242507;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=70-15 / ATCC MYA-4617 / FGSC 8958;
RX   PubMed=15846337; DOI=10.1038/nature03449;
RA   Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K.,
RA   Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D.,
RA   Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S.,
RA   Soanes D.M., Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M.,
RA   Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E.,
RA   Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W.;
RT   "The genome sequence of the rice blast fungus Magnaporthe grisea.";
RL   Nature 434:980-986(2005).
RN   [2]
RP   FUNCTION, DISRUPTION PHENOTYPE, CATALYTIC ACTIVITY, AND SUBCELLULAR
RP   LOCATION.
RX   PubMed=26258762; DOI=10.1038/nchembio.1885;
RA   Patkar R.N., Benke P.I., Qu Z., Chen Y.Y., Yang F., Swarup S., Naqvi N.I.;
RT   "A fungal monooxygenase-derived jasmonate attenuates host innate
RT   immunity.";
RL   Nat. Chem. Biol. 11:733-740(2015).
CC   -!- FUNCTION: Monooxygenase that converts the endogenous (and likely the
CC       host) jasmonate (JA) to its hydroxylated derivative 12-hydroxyjasmonate
CC       (12OH-JA), also known as tuberonic acid, a compound that attenuates or
CC       disables jasmonate-based host innate immunity and which is essential
CC       for proper initiation and elaboration of the blast disease in rice
CC       (PubMed:26258762). ABM, together with a polyketide synthase MGG_04775
CC       and the esterase MGG_04774, share the secondary metabolism gene cluster
CC       with ABC transporter ABC3, and therefore may also be involved in the
CC       synthesis of other important metabolites such as the ABC3 transporter
CC       efflux substrate (ATS) and/or additional polyketides (Probable).
CC       {ECO:0000269|PubMed:26258762, ECO:0000305|PubMed:26258762}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+) + jasmonate + NADPH + O2 = (1R,2R)-12-hydroxyjasmonate +
CC         H2O + NADP(+); Xref=Rhea:RHEA:49984, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58349, ChEBI:CHEBI:58431, ChEBI:CHEBI:132022;
CC         EC=1.14.13.228; Evidence={ECO:0000269|PubMed:26258762};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:49985;
CC         Evidence={ECO:0000269|PubMed:26258762};
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum
CC       {ECO:0000269|PubMed:26258762}. Secreted {ECO:0000269|PubMed:26258762}.
CC       Note=Associates with the cortical endoplasmic reticulum (ER) network
CC       during pre-invasive pathogenic development on the host surface
CC       pathogenesis and remains associated with the cortical ER in the
CC       invasive hyphae untill the later stages of host colonization
CC       (PubMed:26258762). Associates also, albeit weakly, with the biotrophic
CC       interfacial complex (BIC), a specialized secretory organelle present on
CC       the invasive hyphae in M.oryzae (PubMed:26258762). Secreted
CC       specifically during invasive growth (PubMed:26258762).
CC       {ECO:0000269|PubMed:26258762}.
CC   -!- DISRUPTION PHENOTYPE: Fails to develop blast disease on the rice host
CC       but does not affect vegetative growth, asexual development and
CC       appressorium formation (PubMed:26258762). Significantly reduces the
CC       host penetration ability (PubMed:26258762). Leads to the accumulation
CC       of a methylated form of jasmonate (MeJA) resulting in a strong
CC       induction of the host defense response in an otherwise susceptible rice
CC       plant (PubMed:26258762). Does not affect the ability to cause blast
CC       disease in barley (PubMed:26258762). {ECO:0000269|PubMed:26258762}.
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DR   EMBL; CM001231; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; P0CU79; -.
DR   Proteomes; UP000009058; Chromosome 1.
DR   GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   InterPro; IPR007138; ABM_dom.
DR   InterPro; IPR011008; Dimeric_a/b-barrel.
DR   Pfam; PF03992; ABM; 1.
DR   SUPFAM; SSF54909; SSF54909; 1.
PE   1: Evidence at protein level;
KW   Endoplasmic reticulum; Oxidoreductase; Reference proteome; Secreted;
KW   Virulence.
FT   CHAIN           1..215
FT                   /note="Jasmonate monooxygenase ABM"
FT                   /id="PRO_0000446003"
FT   DOMAIN          2..90
FT                   /note="ABM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01062"
SQ   SEQUENCE   215 AA;  24042 MW;  DE74D05BC6C59291 CRC64;
     MFAVIFETRP QPSQFDTYLT IAKSLRPELA NIDGFIENIR YKSLSRPGWI LSLSFWRDEK
     SLVRWRTTAT HHMAQEKGRD GVLEDYHLRV GQVTASVRSN EVKRVETVDA REQDDVTAVG
     AAKTVQLVRF QMDRGAGIDA AAGKLGLNPE EPRGLLAWDI MEAVLSPGDM ILLASWETGS
     SSVSIPGADS DEVQVLRDYG KYDRREAPQF YPPAS