ID   BJAI_BRADU              Reviewed;         219 AA.
AC   Q89VI2;
DT   29-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT   29-MAY-2013, sequence version 2.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=Isovaleryl-homoserine lactone synthase;
DE            Short=IV-HSL synthase;
DE            EC=2.3.1.228;
DE   AltName: Full=Autoinducer synthesis protein BjaI;
GN   Name=bjaI; OrderedLocusNames=blr1063;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, GENE NAME, INDUCTION,
RP   AND DISRUPTION PHENOTYPE.
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=21949379; DOI=10.1073/pnas.1114125108;
RA   Lindemann A., Pessi G., Schaefer A.L., Mattmann M.E., Christensen Q.H.,
RA   Kessler A., Hennecke H., Blackwell H.E., Greenberg E.P., Harwood C.S.;
RT   "Isovaleryl-homoserine lactone, an unusual branched-chain quorum-sensing
RT   signal from the soybean symbiont Bradyrhizobium japonicum.";
RL   Proc. Natl. Acad. Sci. U.S.A. 108:16765-16770(2011).
CC   -!- FUNCTION: Catalyzes the synthesis of IV-HSL (isovaleryl-homoserine
CC       lactone), a quorum-sensing (QS) autoinducer molecule which binds to
CC       BjaR1 transcriptional regulator to activate expression of QS-dependent
CC       genes. Is active with isovaleryl-CoA but cannot use isovaleryl-ACP as
CC       acyl donor. {ECO:0000269|PubMed:21949379}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-methylbutanoyl-CoA + S-adenosyl-L-methionine = CoA + H(+) +
CC         N-isovaleryl-L-homoserine lactone + S-methyl-5'-thioadenosine;
CC         Xref=Rhea:RHEA:37471, ChEBI:CHEBI:15378, ChEBI:CHEBI:17509,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57345, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:74958; EC=2.3.1.228;
CC         Evidence={ECO:0000269|PubMed:21949379};
CC   -!- INDUCTION: By IV-HSL via the transcriptional regulator BjaR1.
CC       {ECO:0000269|PubMed:21949379}.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene fail to produce IV-HSL.
CC       {ECO:0000269|PubMed:21949379}.
CC   -!- SIMILARITY: Belongs to the autoinducer synthase family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00533}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAC46328.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; BA000040; BAC46328.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; NP_767703.1; NC_004463.1.
DR   RefSeq; WP_028172715.1; NZ_CP011360.1.
DR   AlphaFoldDB; Q89VI2; -.
DR   SMR; Q89VI2; -.
DR   STRING; 224911.27349313; -.
DR   EnsemblBacteria; BAC46328; BAC46328; BAC46328.
DR   GeneID; 64020927; -.
DR   KEGG; bja:blr1063; -.
DR   PATRIC; fig|224911.44.peg.458; -.
DR   eggNOG; COG3916; Bacteria.
DR   HOGENOM; CLU_085711_3_0_5; -.
DR   InParanoid; Q89VI2; -.
DR   BRENDA; 2.3.1.228; 14426.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0008080; F:N-acetyltransferase activity; IEA:InterPro.
DR   GO; GO:0009372; P:quorum sensing; IEA:UniProtKB-KW.
DR   GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR   InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR   InterPro; IPR001690; Autoind_synthase.
DR   InterPro; IPR000182; GNAT_dom.
DR   PANTHER; PTHR39322; PTHR39322; 1.
DR   Pfam; PF00765; Autoind_synth; 1.
DR   PRINTS; PR01549; AUTOINDCRSYN.
DR   SUPFAM; SSF55729; SSF55729; 1.
DR   PROSITE; PS51187; AUTOINDUCER_SYNTH_2; 1.
PE   1: Evidence at protein level;
KW   Autoinducer synthesis; Quorum sensing; Reference proteome;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..219
FT                   /note="Isovaleryl-homoserine lactone synthase"
FT                   /id="PRO_0000422390"
SQ   SEQUENCE   219 AA;  25009 MW;  479962A37CE069FC CRC64;
     MIHAISAVNR HLYEDVLEQH FRLRHDIFVE ERHWETLRRP DGREVDSYDD EDTVYLLALE
     GRRVVGGHRL YPTTKPSMMS EVFPHLAAVR GCPSDPLIWE WSRYFVVRDR RDGALNLQLM
     AAVQEFCLDQ GIAQVSAIME TWWLPRFHEA GFVVTPLGLP ALVENAWTMA ATVDIRRQTL
     DVLHDRIGMP SIVQQDGPRL DAVARANLCG LAAAQRKSA