ID   BJAR1_BRADU             Reviewed;         242 AA.
AC   Q89VI3;
DT   29-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 110.
DE   RecName: Full=Transcriptional activator protein BjaR1;
DE   AltName: Full=HTH-type quorum sensing-dependent transcriptional regulator BjaR1;
GN   Name=bjaR1; OrderedLocusNames=blr1062;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
RN   [2]
RP   FUNCTION, GENE NAME, AND DISRUPTION PHENOTYPE.
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=21949379; DOI=10.1073/pnas.1114125108;
RA   Lindemann A., Pessi G., Schaefer A.L., Mattmann M.E., Christensen Q.H.,
RA   Kessler A., Hennecke H., Blackwell H.E., Greenberg E.P., Harwood C.S.;
RT   "Isovaleryl-homoserine lactone, an unusual branched-chain quorum-sensing
RT   signal from the soybean symbiont Bradyrhizobium japonicum.";
RL   Proc. Natl. Acad. Sci. U.S.A. 108:16765-16770(2011).
CC   -!- FUNCTION: Transcriptional activator that functions in response to the
CC       quorum-sensing autoinducer IV-HSL (isovaleryl-homoserine lactone).
CC       Activates BjaI expression. Is sensitive to IV-HSL at concentrations as
CC       low as 10 pM. {ECO:0000269|PubMed:21949379}.
CC   -!- DISRUPTION PHENOTYPE: In cells lacking this gene, bjaI expression is
CC       about 15% of the level in wild-type cells and IV-HSL is <25% of wild-
CC       type levels. {ECO:0000269|PubMed:21949379}.
CC   -!- SIMILARITY: Belongs to the autoinducer-regulated transcriptional
CC       regulatory protein family. {ECO:0000305}.
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DR   EMBL; BA000040; BAC46327.1; -; Genomic_DNA.
DR   RefSeq; NP_767702.1; NC_004463.1.
DR   RefSeq; WP_011083882.1; NZ_CP011360.1.
DR   AlphaFoldDB; Q89VI3; -.
DR   SMR; Q89VI3; -.
DR   STRING; 224911.27349312; -.
DR   EnsemblBacteria; BAC46327; BAC46327; BAC46327.
DR   GeneID; 64020926; -.
DR   KEGG; bja:blr1062; -.
DR   PATRIC; fig|224911.44.peg.457; -.
DR   eggNOG; COG2197; Bacteria.
DR   HOGENOM; CLU_072786_4_1_5; -.
DR   InParanoid; Q89VI3; -.
DR   OMA; NFHIRSV; -.
DR   PhylomeDB; Q89VI3; -.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0009372; P:quorum sensing; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd06170; LuxR_C_like; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 3.30.450.80; -; 1.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR005143; TF_LuxR_autoind-bd_dom.
DR   InterPro; IPR036693; TF_LuxR_autoind-bd_dom_sf.
DR   InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF03472; Autoind_bind; 1.
DR   Pfam; PF00196; GerE; 1.
DR   PRINTS; PR00038; HTHLUXR.
DR   SMART; SM00421; HTH_LUXR; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
DR   SUPFAM; SSF75516; SSF75516; 1.
DR   PROSITE; PS50043; HTH_LUXR_2; 1.
PE   3: Inferred from homology;
KW   Activator; DNA-binding; Quorum sensing; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..242
FT                   /note="Transcriptional activator protein BjaR1"
FT                   /id="PRO_0000422391"
FT   DOMAIN          173..238
FT                   /note="HTH luxR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT   DNA_BIND        197..216
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
SQ   SEQUENCE   242 AA;  27215 MW;  AB48008A044CD015 CRC64;
     MSAVDYGREA LDFIEGLGVY RKVPDAMNAL EAAFGRFGFE TIIVTGLPNP DQRFAQMVLA
     KRWPAGWFNL YTQNNYDRFD PVVRLCRQSV NPFEWSEAPY DAELEPSAAE VMNRAGDFRM
     SRGFIVPIHG LTGYEAAVSL GGVHLDLNPR SKPALHLMAM YGFDHIRRLL EPTPYPSTRL
     TPREREVISW ASQGKSAWEI GEILHITQRT AEEHLATAAR KLGAVNRTHA VALAIRHKII
     NP