BL1S1_YEAST
ID BL1S1_YEAST Reviewed; 122 AA.
AC Q06071; D6VZ42;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Biogenesis of lysosome-related organelles complex 1 subunit BLS1;
DE Short=BLOC-1 subunit BLS1;
DE AltName: Full=BLOS1-homolog;
GN Name=BLS1; OrderedLocusNames=YLR408C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-33, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [7]
RP IDENTIFICATION IN THE BLOC-1 COMPLEX, AND FUNCTION.
RX PubMed=21159114; DOI=10.1111/j.1600-0854.2010.01151.x;
RA Hayes M.J., Bryon K., Satkurunathan J., Levine T.P.;
RT "Yeast homologues of three BLOC-1 subunits highlight KxDL proteins as
RT conserved interactors of BLOC-1.";
RL Traffic 12:260-268(2011).
CC -!- FUNCTION: Component of the biogenesis of lysosome-related organelles
CC complex-1 (BLOC-1), a complex involved in endosomal cargo sorting.
CC {ECO:0000269|PubMed:21159114}.
CC -!- SUBUNIT: Component of the biogenesis of lysosome-related organelles
CC complex-1 (BLOC-1) composed of at least BLI1, BLS1, CNL1, KXD1, SNN1
CC and VAB2. {ECO:0000269|PubMed:21159114}.
CC -!- SUBCELLULAR LOCATION: Endosome {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 468 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the BLOC1S1 family. {ECO:0000305}.
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DR EMBL; U19729; AAB82360.1; -; Genomic_DNA.
DR EMBL; AY558320; AAS56646.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09708.1; -; Genomic_DNA.
DR PIR; S55964; S55964.
DR RefSeq; NP_013512.1; NM_001182296.1.
DR AlphaFoldDB; Q06071; -.
DR SMR; Q06071; -.
DR BioGRID; 31665; 39.
DR ComplexPortal; CPX-1153; BLOC-1 complex.
DR IntAct; Q06071; 5.
DR MINT; Q06071; -.
DR STRING; 4932.YLR408C; -.
DR iPTMnet; Q06071; -.
DR MaxQB; Q06071; -.
DR PaxDb; Q06071; -.
DR PRIDE; Q06071; -.
DR TopDownProteomics; Q06071; -.
DR EnsemblFungi; YLR408C_mRNA; YLR408C; YLR408C.
DR GeneID; 851124; -.
DR KEGG; sce:YLR408C; -.
DR SGD; S000004400; BLS1.
DR VEuPathDB; FungiDB:YLR408C; -.
DR eggNOG; ENOG502S787; Eukaryota.
DR HOGENOM; CLU_150164_0_0_1; -.
DR InParanoid; Q06071; -.
DR OMA; IEANHAY; -.
DR BioCyc; YEAST:G3O-32470-MON; -.
DR PRO; PR:Q06071; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; Q06071; protein.
DR GO; GO:0031083; C:BLOC-1 complex; IDA:SGD.
DR GO; GO:0005768; C:endosome; IDA:ComplexPortal.
DR GO; GO:0007032; P:endosome organization; IMP:SGD.
DR GO; GO:0032880; P:regulation of protein localization; IMP:SGD.
PE 1: Evidence at protein level;
KW Endosome; Phosphoprotein; Reference proteome; Transport.
FT CHAIN 1..122
FT /note="Biogenesis of lysosome-related organelles complex 1
FT subunit BLS1"
FT /id="PRO_0000247218"
FT MOD_RES 33
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
SQ SEQUENCE 122 AA; 14549 MW; 0B3BEA4E33149248 CRC64;
MFLTFSMCVN WIIVKMPNRS EELDRLLDKI INSPHRTEAS KTLQEIENNQ SYILNVQLKK
LLRLHDDSFK NKCVSPINYM LEKYTPYMGH TEALQKEAEL VDRDLRILEM TYQLIEKNRN
SK
