ID   SYGB_ACIAC              Reviewed;         708 AA.
AC   A1TUT0;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=Aave_4177;
OS   Acidovorax citrulli (strain AAC00-1) (Acidovorax avenae subsp. citrulli).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Acidovorax.
OX   NCBI_TaxID=397945;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AAC00-1;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T.,
RA   Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P.;
RT   "Complete sequence of Acidovorax avenae subsp. citrulli AAC00-1.";
RL   Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000512; ABM34718.1; -; Genomic_DNA.
DR   RefSeq; WP_011797192.1; NC_008752.1.
DR   AlphaFoldDB; A1TUT0; -.
DR   SMR; A1TUT0; -.
DR   STRING; 397945.Aave_4177; -.
DR   EnsemblBacteria; ABM34718; ABM34718; Aave_4177.
DR   KEGG; aav:Aave_4177; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_4; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000002596; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..708
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101252"
SQ   SEQUENCE   708 AA;  75937 MW;  8E7ED29582814D80 CRC64;
     MTHPNLLVEL FVEELPPKAL QKLGDAFAGV LLAQLQAQGL ASAASRVTAY ASPRRLAAHV
     TEVAPQAADK AVSQKLMPVT VGLDASGNAT PALLKKLAAL GADASAVPGL RRAPDGKAEA
     LFLDSTVRGA TLSEGLQKAL QESVAKLPIP KVMRYQLSSG CEQPGWTSVS FVRPAHGLVA
     LHGTEVLLSV SVLGLTAGNA THGHRFEAAV DPVVIRSADT YAQQLAEEGA VIAAFADRRA
     EIARQLQAAA EQVGGGVRPI QDDALLDEVT ALVERPNVLV CEFEKQFLDV PQECLILTMK
     ANQKYFPLLD AEGRLTHRFL VVSNIRPDDA SAVVGGNERV VRPRLADAKF FFDQDRKKTL
     ESRVASLAKV VYHNQLGTQG ERVERVRAIA RAIAQQIGDV ELARQADQAA QLAKADLVTD
     MVGEFPELQG TMGRYYAQAD GLPAEVADAI EDHYKPRFAG DTLPRGDVGV VVALADKLET
     LVGMFGIGNL PTGDRDPFAL RRHALGVIRM LVEKELPLDL ETLLHSALPA FGDKIQDPTA
     SLADFIYDRL AGSLREQGYS AQEVDAVLAL RPQRLALVGK QLAAVRAFAA LPEAPALAAA
     NKRVTNILKK AGDVDAHVNP DLLREQAEKD LYAALQRFVP EADALFAAGD YTGSLQTLAV
     LRAPVDAFFD DVMVNAEELD LRLNRQGLLQ SLHVAMNRVA DLSRLAVA