ID   SYGB_ACIET              Reviewed;         720 AA.
AC   B9MCM4;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 70.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=Dtpsy_0544;
OS   Acidovorax ebreus (strain TPSY) (Diaphorobacter sp. (strain TPSY)).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Diaphorobacter.
OX   NCBI_TaxID=535289;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TPSY;
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D.,
RA   Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Coates J.D.;
RT   "Complete sequence of Diaphorobacter sp. TPSY.";
RL   Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP001392; ACM32024.1; -; Genomic_DNA.
DR   RefSeq; WP_012655567.1; NC_011992.1.
DR   AlphaFoldDB; B9MCM4; -.
DR   SMR; B9MCM4; -.
DR   STRING; 535289.Dtpsy_0544; -.
DR   EnsemblBacteria; ACM32024; ACM32024; Dtpsy_0544.
DR   KEGG; dia:Dtpsy_0544; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_4; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000000450; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..720
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000197183"
SQ   SEQUENCE   720 AA;  77923 MW;  22FC6F12D3EBC93D CRC64;
     MNHQNLLVEL FVEELPPKAL QKLGDAFAGV LLEQLQAQGL TSAHSQLTAF ASPRRLAAHI
     TEVLPAAADK AVSQKLMPVA VGLDASGQPT PALLKKLAAL GADAASVPQL KRVHDGKAEV
     LFFESMAKGA LLADGLQKAL DEAIAKLPIP KVMRYQLQDG WTSVHFVRPA HGLVALHGSE
     VLVGVQALGL TAGNTTHGHR FEASVDPVVI QSADSYAEQL RSEGAVIASF AERRAEIARQ
     LQAAADRVGG GVRPIDDDAL LDEVTALVER PNVLVCEFEK DFLAVPQECL ILTMKANQKY
     FPLLDAEGKL THQFLVVSNI SPQDASAVIQ GNERVVRPRL ADAKFFFDQD RKKTLVSRVD
     QLAKVVYHNK LGTQGERVER VRHIAKAIAT QLFTALAQGN AALDSQEGEI AQDYLLTCVD
     NAALLAKTDL VTDMVGEFPE LQGIMGGYYA VSDGLPDEVA HAIEDHYKPR FAGDALPREN
     VGVVVALADK LETLVGMFGI GNLPTGDRDP FALRRHALGV IRMLVEKELP LDLDALLASA
     VPAFGDKIED TSAQLADFIY DRLAGSLREQ GYSAQEVDAV IALRPQRLAL VPRQIEAVRA
     FATLEQAPAL AAANKRVTNI LKKAGEVDPH VNEELLQEPA EKDLYAALQR FVPEANAQFD
     SGDYTASLQT LAVLRAPVDA FFDDVMVNAE ELALRLNRQG LLKKLHMAMN RVADLSRLAV