ID   SYGB_ACISJ              Reviewed;         720 AA.
AC   A1W3F3;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=Ajs_0528;
OS   Acidovorax sp. (strain JS42).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Acidovorax; unclassified Acidovorax.
OX   NCBI_TaxID=232721;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JS42;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T.,
RA   Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P.;
RT   "Complete sequence of chromosome 1 of Acidovorax sp. JS42.";
RL   Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000539; ABM40778.1; -; Genomic_DNA.
DR   RefSeq; WP_011803987.1; NC_008782.1.
DR   AlphaFoldDB; A1W3F3; -.
DR   SMR; A1W3F3; -.
DR   STRING; 232721.Ajs_0528; -.
DR   EnsemblBacteria; ABM40778; ABM40778; Ajs_0528.
DR   KEGG; ajs:Ajs_0528; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_4; -.
DR   OMA; LPIPKRM; -.
DR   Proteomes; UP000000645; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..720
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101258"
SQ   SEQUENCE   720 AA;  78008 MW;  70922925A521CF86 CRC64;
     MNHQNLLVEL FVEELPPKAL QKLGDAFAGV LLEQLQAQGL TSAHSQLTAF ASPRRLAAHI
     TEVLPAAADK AVSQKLMPVA VGLDASGQPT PALLKKLTAL GADAASVPQL KRVHDGKAEV
     LFFESMAKGA LLADGLQKAL DEAIAKLPIP KVMRYQLQDG WTSVHFVRPA HGLVALHGTQ
     VLVGVQALGL TAGNTTHGHR FEASVDPVVI QSADSYAEQL RSEGAVIASF AERRAEIARQ
     LQAAADRVGG GVRPIEDAAL LDEVTALVER PNVLVCEFEK EFLAVPQECL ILTMKANQKY
     FPLLDAEGKL THQFLVVSNI SPQDASAVIQ GNERVVRPRL ADAKFFFDQD RKKTLVSRVD
     QLAKVVYHNK LGTQGERVER VRHIAKAIAT QLFTALAQGN AALDSQEGEI AQDYLLTCVD
     NAALLAKTDL VTDMVGEFPE LQGIMGGYYA VSDGLPDEVA HAIEDHYKPR FAGDALPREN
     VGVVVALADK LETLVGMFGI GNLPTGDRDP FALRRHALGV IRMLVEKELP LDLDALLASA
     VPAFGDKIED TSAQLADFIY DRLAGSLREQ GYSAQEVDAV IALRPQRLAL VPRQLEAVRA
     FTQLEEAPAL AAANKRVTNI LKKAGEVDPH VNEELLQEPA EKDLYAALQR FVPEANAQFD
     SGDYTASLQT LAVLRAPVDA FFDDVMVNAE ELALRLNRQG LLKKLHMAMN RVADLSRLAV