ID   SYGB_ACTSZ              Reviewed;         689 AA.
AC   A6VLE1;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=Asuc_0410;
OS   Actinobacillus succinogenes (strain ATCC 55618 / DSM 22257 / CCUG 43843 /
OS   130Z).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Actinobacillus.
OX   NCBI_TaxID=339671;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 55618 / DSM 22257 / CCUG 43843 / 130Z;
RX   PubMed=21118570; DOI=10.1186/1471-2164-11-680;
RA   McKinlay J.B., Laivenieks M., Schindler B.D., McKinlay A.A.,
RA   Siddaramappa S., Challacombe J.F., Lowry S.R., Clum A., Lapidus A.L.,
RA   Burkhart K.B., Harkins V., Vieille C.;
RT   "A genomic perspective on the potential of Actinobacillus succinogenes for
RT   industrial succinate production.";
RL   BMC Genomics 11:680-680(2010).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000746; ABR73788.1; -; Genomic_DNA.
DR   RefSeq; WP_012072173.1; NC_009655.1.
DR   AlphaFoldDB; A6VLE1; -.
DR   SMR; A6VLE1; -.
DR   STRING; 339671.Asuc_0410; -.
DR   EnsemblBacteria; ABR73788; ABR73788; Asuc_0410.
DR   KEGG; asu:Asuc_0410; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_6; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000001114; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..689
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000071878"
SQ   SEQUENCE   689 AA;  76155 MW;  9F4FE9A284EB10C0 CRC64;
     MTTENFFAEI GTEELPPKAL KKLATAFAEN VENELKQAGL AFDKVEWFAA PRRLAVKVLN
     LAANQPSKEI EKRGPAVSAA FDAEGKPTKA AEGWARGCGI TVDQAERLVT DKGEWLVHRA
     VIEGQPTKNL MLDIIAKSLA NLPIPKTMRW GDKNVQFVRP VHTVTLLLGG ELIEGEILGI
     ASGRTIRGHR FLGEREFQIS HADQYPSLLE EKGCVIADFN KRREMILTKS QEKATALGGV
     ADIEDDLLDE VTSLVEFPNV LTAKFEERFL AVPAEALVYT MKGDQKYFPI YDKEGKLLPH
     FIFVSNINPV DPMPIIEGNE KVVRPRLSDA EFFFNTDKKQ RLEDLLPRLQ TVLFQQQLGT
     LLDKTKRIQS LAGEIAGQIG ADKAKAERAG LLSKCDLMTN MVFEFTDTQG VMGMHYARHD
     GEDEEVAVAL NEQYMPRFAG DNLPNSLVAS SVALADKFDT LTGIFGIGQA PKGSADPFAL
     RRAALGALRI IVEKNLSLDL ADLVKKSAAL FGDKLTNANV VDDVVDFMLG RFRAWYQDGG
     IAVDVIQAVL ARRPTKPADF DARVRAVSHF RTLDSAEALA AANKRVSNIL AKVAGEIRSE
     IDRTLLLEAE EKALAEQVLA LQSELAPVFA KGDYQTALDR LACLREVVDN FFDKVMVNAE
     DEKLRQNRQA MLNVLRNLFL QVADISLLQ