ID   SYGB_AERHH              Reviewed;         689 AA.
AC   A0KEJ9;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-DEC-2006, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=AHA_0127;
OS   Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / BCRC
OS   13018 / CCUG 14551 / JCM 1027 / KCTC 2358 / NCIMB 9240 / NCTC 8049).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales;
OC   Aeromonadaceae; Aeromonas.
OX   NCBI_TaxID=380703;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 7966 / DSM 30187 / BCRC 13018 / CCUG 14551 / JCM 1027 / KCTC
RC   2358 / NCIMB 9240 / NCTC 8049;
RX   PubMed=16980456; DOI=10.1128/jb.00621-06;
RA   Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H.,
RA   Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S.,
RA   Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F.;
RT   "Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades.";
RL   J. Bacteriol. 188:8272-8282(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000462; ABK37089.1; -; Genomic_DNA.
DR   RefSeq; WP_011704155.1; NC_008570.1.
DR   RefSeq; YP_854662.1; NC_008570.1.
DR   AlphaFoldDB; A0KEJ9; -.
DR   SMR; A0KEJ9; -.
DR   STRING; 380703.AHA_0127; -.
DR   PRIDE; A0KEJ9; -.
DR   EnsemblBacteria; ABK37089; ABK37089; AHA_0127.
DR   KEGG; aha:AHA_0127; -.
DR   PATRIC; fig|380703.7.peg.120; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_6; -.
DR   OMA; LPIPKRM; -.
DR   Proteomes; UP000000756; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   SMART; SM00836; DALR_1; 1.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..689
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000006349"
SQ   SEQUENCE   689 AA;  75202 MW;  E895642BBA6F5D0D CRC64;
     MAQHTFLVEI GTAELPPKAL RSLAEAFADN LKSELTKADL AFGDVEWFAS PRRLALKVSE
     LAGEQPSKSV EKRGPAVAQA FDAEGKPTKA AEGWARGNGI TVEQAERLVT DKGEWLVHTA
     KVEGRPAKDL LGELVSQALA KLPIPKMMRW GDKTIQFVRP VFTVTLLLDG ELVPAHILGI
     DSARTLRGHR FMGESEFTID NASQYPQILQ ERGMVIADFM ARKVKIKADA EAAAAAFGGV
     ADLDDALLEE VTALVEWPVV LTANFEEKFL AVPAEALVHT MKGDQKYFPV YDKNGKLLPK
     FIFVTNIESK DPSQIISGNE KVVRPRLSDA EFFFKTDLKQ TLASRLPRLE TVLFQQQLGT
     VKAKVERIET VAGFIAERIG ADVAQAKRAG LLSKCDLMTN MVGEFTDTQG VMGMHYARHD
     GEDEAVAVAL NEQYMPRFAG DALPSGLVAC AVALADKFDT LAGIFGIGML PKGDKDPFAL
     RRAAIGALRI MTEKQLDLDL VELVEEAVRV YGDKLTNKTV VTDVVDFMLG RFRAAYQDEG
     IGADVVLAVL ARRPTRPLDF DRRVKAVSHF RSLDAALALA AANKRVSNIL AKVEGELPTA
     VKPELLVDAA EKALATQVAE LQAELAPLFA AGDYQAALTR LAALREPVDT FFNEVMVMAD
     DEALKANRLA LLNNLRNLFL QVADISLLQ