ID   SYGB_AERS4              Reviewed;         689 AA.
AC   A4STG3;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   15-MAY-2007, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=ASA_4261;
OS   Aeromonas salmonicida (strain A449).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales;
OC   Aeromonadaceae; Aeromonas.
OX   NCBI_TaxID=382245;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=A449;
RX   PubMed=18801193; DOI=10.1186/1471-2164-9-427;
RA   Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J.,
RA   Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C.,
RA   Brown L.L.;
RT   "The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into
RT   the evolution of a fish pathogen.";
RL   BMC Genomics 9:427-427(2008).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000644; ABO92185.1; -; Genomic_DNA.
DR   RefSeq; WP_005320601.1; NC_009348.1.
DR   AlphaFoldDB; A4STG3; -.
DR   SMR; A4STG3; -.
DR   STRING; 382245.ASA_4261; -.
DR   EnsemblBacteria; ABO92185; ABO92185; ASA_4261.
DR   KEGG; asa:ASA_4261; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_6; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000000225; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   SMART; SM00836; DALR_1; 1.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..689
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000006350"
SQ   SEQUENCE   689 AA;  75145 MW;  BC1CCED6F64DAA59 CRC64;
     MAQHTFLVEI GTAELPPKAL RSLAEAFADN FKAELTKADL AFGDIEWFAS PRRLALKVHA
     LASEQPSKSV EKRGPAVSQA FDAEGKPTKA AEGWARGNGI TVEQAERLVT DKGEWLVHTA
     KVEGRPAKDL LGELVASALA KLPIPKMMRW GDKTIQFVRP VFTVTLLLDG ELVPAHILGI
     DSARTIRGHR FMGEPEFTID NASQYPQILL EKGKVVADFM ARKAKIKADA EAAAAAFGGV
     ADLDDALLEE VTALVEWPVV LTANFEEKFL AVPAEALVHT MKGDQKYFPV YDKNGKLLPK
     FIFVTNIESK DPSQIISGNE KVVRPRLSDA EFFFKTDLKQ TLASRLPRLE TVLFQQQLGT
     VKAKVERIET VAGFIAERIG ANVAQAKRAG LLSKCDLMTN MVGEFASTQG VMGMHYARHD
     GEDEVVAVAL NEQYMPRFAG DALPSALEAC AVALADKLDT LAGIFGIGML PKGDKDPFAL
     RRAAIGALRI MTEKQLDLDL VELVEEAVRV YGDKLTNKTV VTDVVDFMLG RFRAAYQDEG
     IGADVVLAVL ARRPTRPLDF DRRVKAVSHF RSLDAALALA AANKRVSNIL AKVEGELPTA
     VKPELLQDAA EKALATQVAE LQAELAPLFA AGDYQAALTR LAALREPVDT FFNEVMVMAD
     DEALKANRLA LLNNLRNLFL QVADISLLQ