SYGB_ALIF1
ID SYGB_ALIF1 Reviewed; 688 AA.
AC Q5E8Y5;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2005, sequence version 1.
DT 25-MAY-2022, entry version 105.
DE RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=VF_0016;
OS Aliivibrio fischeri (strain ATCC 700601 / ES114) (Vibrio fischeri).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Aliivibrio.
OX NCBI_TaxID=312309;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700601 / ES114;
RX PubMed=15703294; DOI=10.1073/pnas.0409900102;
RA Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R.,
RA Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E.,
RA Stevens A., Visick K., Whistler C., Greenberg E.P.;
RT "Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with
RT pathogenic congeners.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC {ECO:0000255|HAMAP-Rule:MF_00255}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR EMBL; CP000020; AAW84511.1; -; Genomic_DNA.
DR RefSeq; WP_011260903.1; NC_006840.2.
DR RefSeq; YP_203399.1; NC_006840.2.
DR AlphaFoldDB; Q5E8Y5; -.
DR SMR; Q5E8Y5; -.
DR STRING; 312309.VF_0016; -.
DR EnsemblBacteria; AAW84511; AAW84511; VF_0016.
DR KEGG; vfi:VF_0016; -.
DR PATRIC; fig|312309.11.peg.17; -.
DR eggNOG; COG0751; Bacteria.
DR HOGENOM; CLU_007220_2_2_6; -.
DR OMA; LPIPKRM; -.
DR OrthoDB; 213210at2; -.
DR Proteomes; UP000000537; Chromosome I.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR InterPro; IPR008909; DALR_anticod-bd.
DR InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR PANTHER; PTHR30075; PTHR30075; 1.
DR Pfam; PF05746; DALR_1; 1.
DR Pfam; PF02092; tRNA_synt_2f; 1.
DR PRINTS; PR01045; TRNASYNTHGB.
DR SMART; SM00836; DALR_1; 1.
DR TIGRFAMs; TIGR00211; glyS; 1.
DR PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT CHAIN 1..688
FT /note="Glycine--tRNA ligase beta subunit"
FT /id="PRO_1000006419"
SQ SEQUENCE 688 AA; 76041 MW; F316CCAEB6DBD57B CRC64;
MAKNFLIELG TEELPPTALR SLAEAFASNF EAELKAADLA HQGVKWYATP RRLALKVAEL
AESQADKVVE KRGPAVSAAF DADGNPTKAA QGWARGNGIT VEQADRLKTD KGEWLLHKEE
VKGKPVQELV VDFAAKALAG LPIPKAMRWG NSDIQFIRPV KTLTILLGDE LIEGSILGVS
SARTLRGHRF MGESEFTIDS ADQYPAILEE RGKVMADYDA RKAIILADSE KAAAAVGGKA
DLEDDLVEEV TSLVEWPVVL TAKFEEEFLK VPSEALVYTM KGDQKYFPVY DENKKLLPNF
IFVSNIESKE PRHVIEGNEK VVRPRLADAE FFFNTDRKRP LIDRLPELEQ AIFQKQLGTI
KDKTDRITEL AGYIAEQIGA DVEKSQRAGL LAKCDLMTSM VFEFTDTQGV MGMHYATHDG
EDEQVALALY EQYMPRFAGD DLPSTDISSA VAMADKLDTL VGIFGIGQAP KGSDPFALRR
AALGVLRIIV EKEYNLDLVD LVAKAQSLFG DKLSNANVAT DVIDFMLGRF RAWYQDEGFS
VDIIQAVLAR RPTKPADFDK RVKAVSHFRE LDAAESLAAA NKRVGNILAK FDGELAQEID
LALLQEDAEK ALAEKVEILA EALEPVFAAG NYQEALSRLA ELREPVDAFF DNVMVMADDE
ALKTNRLTLL NKLRNLFLDI ADISLLQK