ID   SYGB_ALISL              Reviewed;         688 AA.
AC   B6EGT3;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   25-NOV-2008, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=VSAL_I2965;
OS   Aliivibrio salmonicida (strain LFI1238) (Vibrio salmonicida (strain
OS   LFI1238)).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Aliivibrio.
OX   NCBI_TaxID=316275;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LFI1238;
RX   PubMed=19099551; DOI=10.1186/1471-2164-9-616;
RA   Hjerde E., Lorentzen M.S., Holden M.T., Seeger K., Paulsen S., Bason N.,
RA   Churcher C., Harris D., Norbertczak H., Quail M.A., Sanders S.,
RA   Thurston S., Parkhill J., Willassen N.P., Thomson N.R.;
RT   "The genome sequence of the fish pathogen Aliivibrio salmonicida strain
RT   LFI1238 shows extensive evidence of gene decay.";
RL   BMC Genomics 9:616-616(2008).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; FM178379; CAQ80649.1; -; Genomic_DNA.
DR   RefSeq; WP_012551367.1; NC_011312.1.
DR   AlphaFoldDB; B6EGT3; -.
DR   SMR; B6EGT3; -.
DR   STRING; 316275.VSAL_I2965; -.
DR   EnsemblBacteria; CAQ80649; CAQ80649; VSAL_I2965.
DR   KEGG; vsa:VSAL_I2965; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_6; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000001730; Chromosome 1.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..688
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101262"
SQ   SEQUENCE   688 AA;  75890 MW;  E69F45D8811383C0 CRC64;
     MAKNFLIELG TEELPPKALR SLAEAFAANF EAGLKAAGLA HQGIKWYATP RRLALKIAEL
     DEGQADKIVE KRGPAIASAF DADGNPTKAA QGWARGNGIT VEQAERLKTD KGEWLLHKEE
     VKGQPVKGLV VELAAKALAG LPIPKAMRWG NSDIQFIRPV KTLTILLGDE LIEGTILGVA
     STRTIRGHRF MGESEFTIDS ADQYPAILEE RGKVMADYDA RKAIILAGAK KAAEAVGGIA
     DLEDELVEEV TSLVEWPVVL TAKFEQEFLN VPSEALVYTM KGDQKYFPVY DQEKNLLPNF
     IFVTNIESKE PRHIIEGNEK VVRPRLADAE FFFNTDRKRP LIDRLPELEQ AIFQKQLGTI
     KDKTDRITEL AGYIAEQIGA DVEKSQRAGL LAKCDLMTSM VFEFTDTQGV MGMHYATHDG
     EDAQVALALY EQYMPRFAGD DLPSTDVSAS VAMADKLDTL VGIFGIGQAP KGSDPFALRR
     AALGILRIIV EKGYNLDLVD LVAKAQSLFG DKLTNANVDT DVIDFMLGRF RAWYQDEGFS
     VDIIQAVLAR RPTKPADFDQ RVKAVSHFRE LDAAESLAAA NKRVGNILAK FDGELAQEID
     LALLQEDAEK VLAEKVEILA EALEPVFIAG NYQEALSRLA ELREPVDAFF DGVMVMADDE
     ALKLNRLTLL NKLRNLFLDI ADISLLQK