ID   SYGB_ALKCK              Reviewed;         693 AA.
AC   Q5WHD5;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   25-MAY-2022, entry version 109.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=ABC1685;
OS   Alkalihalobacillus clausii (strain KSM-K16) (Bacillus clausii).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalobacillus.
OX   NCBI_TaxID=66692;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KSM-K16;
RA   Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y.,
RA   Kawai S., Ito S., Horikoshi K.;
RT   "The complete genome sequence of the alkaliphilic Bacillus clausii KSM-
RT   K16.";
RL   Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AP006627; BAD64220.1; -; Genomic_DNA.
DR   RefSeq; WP_011246529.1; NC_006582.1.
DR   AlphaFoldDB; Q5WHD5; -.
DR   SMR; Q5WHD5; -.
DR   STRING; 66692.ABC1685; -.
DR   EnsemblBacteria; BAD64220; BAD64220; ABC1685.
DR   KEGG; bcl:ABC1685; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_9; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000001168; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..693
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000006351"
SQ   SEQUENCE   693 AA;  77516 MW;  09D34ADE6676A473 CRC64;
     MNKPFLLELG LEELPARFVT PSITQLAEKV QAWLDDKGLS YGEIHSYATP RRLAILVENL
     ADRQPDIHDE SRGPALKIAK DESGAWTKAA LGFAKGQGVE TSQLEIKAVN GTDYVFAKTH
     KKGEKTSVLL PELKELITGL TFPKSMRWNT YSLRYARPIQ WLVALYGEDV IPFSINGIET
     GRNTTGHRFL GADFPLDNPI EYADALKRQY VIADAEERKA AIVSQLKQME EEHSWVVPID
     EALLEEVTQL VEYPTALSGS FDDSFLALPK EVLITTMREH QRYFPVEAKD GRLLPYFITV
     RNGNEEYIEN VAKGNEKVLR ARLSDAAFFY HEDQKISLQA ANAKLDNIVF HEAIGTMGEK
     VARIGELSKW LAGEANLGAQ ERQTVERAAA IAKFDLVTYM VGEFPELQGR MGQEYAEKAG
     ETPQVAKAIY EQYLPRYAGD KVPETEAGAV LALADKLDTV VSCFSIGLIP TGSQDPYALR
     RQATGIIHIL LQTEMPLTLE NMAVHALDSI AKKGFMTEKV ETTKQELISF FANRLKHVML
     EAGFRYDIVD AVLAAPLVDV YTMFAKARLL TERAEDREFK EVTESLSRVT NLAKKAPKGV
     FVSENLFENE TESRLLAATL EVEFGLAEAW KVKDAYAAYQ ALAQCRATIN TFFDHTMVMA
     DNEATRNNRL ALLDRLAKSI QSYADFQKIV FSA