ID   SYGB_ALTMD              Reviewed;         692 AA.
AC   B4S277; F2G1Q9;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   OrderedLocusNames=MADE_1000035;
OS   Alteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep
OS   ecotype).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Alteromonadaceae; Alteromonas/Salinimonas group; Alteromonas.
OX   NCBI_TaxID=1774373;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype;
RX   PubMed=18670397; DOI=10.1038/ismej.2008.74;
RA   Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S.,
RA   Johnson J., Friedman R., Rodriguez-Valera F.;
RT   "Comparative genomics of two ecotypes of the marine planktonic copiotroph
RT   Alteromonas macleodii suggests alternative lifestyles associated with
RT   different kinds of particulate organic matter.";
RL   ISME J. 2:1194-1212(2008).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP001103; AEA96156.1; -; Genomic_DNA.
DR   RefSeq; WP_012516530.1; NC_011138.3.
DR   AlphaFoldDB; B4S277; -.
DR   SMR; B4S277; -.
DR   EnsemblBacteria; AEA96156; AEA96156; MADE_1000035.
DR   KEGG; amc:MADE_1000035; -.
DR   PATRIC; fig|314275.5.peg.9; -.
DR   HOGENOM; CLU_007220_2_2_6; -.
DR   OMA; LPIPKRM; -.
DR   Proteomes; UP000001870; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   SMART; SM00836; DALR_1; 1.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..692
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101263"
SQ   SEQUENCE   692 AA;  75714 MW;  50A72F3CA4B78C65 CRC64;
     MRTENCLVEL GTEELPPKAL KSLGEAFATQ FEAALSQADL SFDSVSWFAA PRRLAVYVSG
     LAEGQADKVV EKRGPAVSAA FDADGNPTKA AQGWARGNGI DVADAERLVT DKGEWLLHKA
     HVPGQSVAEL LEGLINQAVS KLPIPKPMRW GNYNTQFIRP VHTLCVLYGS EVVNVSVLGL
     TSGRVVQGHR FHGEGRFELD HADNYASALE QQYVLADFEA RKDKVRQQLE DAAKNLSLKP
     DYDEELLEEI ASLVEWPVVL QAGFDKGFLE VPKEALIYTM KDDQKYVPLL DSDGALSNTF
     LFVTNIESHD ASQVISGNEK VIRPRLADAE FFFNSDKKTT LESRLESLET VLFQKQLGTL
     KEKSERISAL SAFIASQIDA NETQAARAGL LAKTDLMSNM VMEFPDVQGV MGKYYALNDG
     EDAPVAEALY EQYMPRFAGD ALPSSGVSAS VALADKLDTL VGIFGIGQLP KGDKDPFALR
     RAAIGVLRIV TELSLPLDLE TLVSKAIDVY GNKLTNAETQ TQVVDFVLGR FNALLQDQAI
     AIDVIQAVAA RRPTKPSDYL ARVHAVDKFK ALEEAEALAA ANKRVANILA KQNVEVTATV
     NIDESLLAED AEKALYVELK AAQKEVEIAV PSQDYTRILT TLATLRNVID NFFDNVMVMA
     DDEAVKHNRL ALLSLLRQLF LTTADISILA KS