ID   SYGB_ANASK              Reviewed;         701 AA.
AC   B4UG87;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=AnaeK_2554;
OS   Anaeromyxobacter sp. (strain K).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales;
OC   Cystobacterineae; Anaeromyxobacteraceae; Anaeromyxobacter;
OC   unclassified Anaeromyxobacter.
OX   NCBI_TaxID=447217;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K;
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C.,
RA   Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikiva G.,
RA   Beliaev A.;
RT   "Complete sequence of Anaeromyxobacter sp. K.";
RL   Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP001131; ACG73779.1; -; Genomic_DNA.
DR   RefSeq; WP_012526563.1; NC_011145.1.
DR   AlphaFoldDB; B4UG87; -.
DR   SMR; B4UG87; -.
DR   EnsemblBacteria; ACG73779; ACG73779; AnaeK_2554.
DR   KEGG; ank:AnaeK_2554; -.
DR   HOGENOM; CLU_007220_2_2_7; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000001871; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   SMART; SM00836; DALR_1; 1.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..701
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000197168"
SQ   SEQUENCE   701 AA;  75745 MW;  391A2CA4F2EA58BF CRC64;
     MADLLFEIGA EEIPAGFVPG ALRQLEDDLA KALADARLAH GEVRSVGTPR RLAVWARDVA
     PKQTDARTEA FGPPVAQAYD AEGKPTPAAT GFARSQGVEV SALVRAQTPK GERVAVTKVE
     KGRRAEQVLP ALLERLVGGL RFRKAMRSRF DEATFARPVR WMVALLGGRP LKVRHGEVTS
     GKVTYGHRFL APKAIALKGT PDDYLAKLRR AHVLADPVER RAALLAELAR AGKEAAGKVR
     EDPALVEQVL YLVEEPTAVV GEFEKSNLEL PPEVVISEMR NHQRYFAVVD GKGRLKNRFV
     AVSATRVKDP AVARHGYERV LRARLADARF FFEEDRKRKL HERIEDLGRR TFQAKLGSEL
     DRAQRIGAVA SALARALGKD ALVADLLEAS RLAKVDLNTG MVGEFPELQG TMGAHYARLE
     GLKPEIADAI EDHYKPIGAA EELPRSDLGA LVAVADRLHS LVGIIGVGEK ATGAADPFGL
     RRAAIGILRI VIARGYHLSL AAAVEQTLDA LSGVKLAAGR ALVAEQVLDF LRGRVRAAWT
     ERFDADLVEA VLAAGSDDVV DARRRLEALA DAKARPDFGS LAVAFKRVAN IQEKAGGSGA
     AAVDPALLRD AAEKDLLAAL EKVEQEVVAR RAARDYPAVL RTVATLEPAV ARFFDGVLVM
     AEDPALRANR LGLMRRVAAL FSDLADFRKI QAEAPAQARA G