ID   SYGB_AQUAE              Reviewed;         664 AA.
AC   O67898;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 123.
DE   RecName: Full=Glycine--tRNA ligase beta subunit;
DE            EC=6.1.1.14;
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit;
DE            Short=GlyRS;
GN   Name=glyS; OrderedLocusNames=aq_2141;
OS   Aquifex aeolicus (strain VF5).
OC   Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex.
OX   NCBI_TaxID=224324;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=VF5;
RX   PubMed=9537320; DOI=10.1038/32831;
RA   Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA   Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA   Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT   "The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.";
RL   Nature 392:353-358(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14;
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE000657; AAC07870.1; -; Genomic_DNA.
DR   PIR; F70483; F70483.
DR   RefSeq; NP_214467.1; NC_000918.1.
DR   RefSeq; WP_010881403.1; NC_000918.1.
DR   AlphaFoldDB; O67898; -.
DR   SMR; O67898; -.
DR   STRING; 224324.aq_2141; -.
DR   PRIDE; O67898; -.
DR   EnsemblBacteria; AAC07870; AAC07870; aq_2141.
DR   KEGG; aae:aq_2141; -.
DR   PATRIC; fig|224324.8.peg.1655; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_0; -.
DR   InParanoid; O67898; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000000798; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..664
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_0000072892"
SQ   SEQUENCE   664 AA;  77524 MW;  7FFEF7A8F552E3DB CRC64;
     MTNELLIEIG TEELPAGVIN PALDYLKDKI NSLLNARQVK TYGTPRRLTL YFKDFENERK
     EKKEVIWGPP KNVAYDEKGN PTKALEGFLK KNNASLEEVK VLKKDKGEYV AIVRKVIEKS
     PIEKLQEEFE EILLSVPFPK RMRWTSSKRI TFSRPVRWIL ALFNGQVLKL RFGELESSNK
     TYGHRFLSKG EVTINNPADY EKTLKEHYVI PDFNERKEII LRALEKSSQE VGGKPSYPEG
     LVEEVTNLVE YPFPVLGKFD EKYLELPPLV ITTVAAHHQR FFCFEKDGKL LNYFLGISNN
     KPNEKIKEGY EKVLRARLED ALFFYREDLK KDLKSLIPEL KKVLFHPKVG SMYEKEERME
     KIAQKLCPLL KCEWEKVKEA VWLSKVDLLT EMVKELDELQ GYMGYVYAKA QGYDEEVAKA
     LWEQYFPRSL EDKVPETTTG TILSLSDKID NLYSFFKAGE IPKGSSDPYG LRRSAFGIIK
     IVDVKNLDLN LEDFKEIYGE FKQYPKLVEF LKQRLISYLE DYPVDIVRAV LNVYSPMEPY
     KVINSVRVLY EASKSPEFPS VVEAAKRVIR IIPKDWKNYE VDEKLLSEEA ERELYQKLTE
     FENKELKSPL ELLPLKEYID KFFDNVKVMA EDEKIRNNRI SLLKRVENLF RTFGDFNEIV
     IKEG