ID   SYGB_AROAE              Reviewed;         696 AA.
AC   Q5P766;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   04-JAN-2005, sequence version 1.
DT   25-MAY-2022, entry version 110.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=AZOSEA07220;
GN   ORFNames=ebA1330;
OS   Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales;
OC   Rhodocyclaceae; Aromatoleum.
OX   NCBI_TaxID=76114;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=EbN1;
RX   PubMed=15551059; DOI=10.1007/s00203-004-0742-9;
RA   Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F.,
RA   Reinhardt R.;
RT   "The genome sequence of an anaerobic aromatic-degrading denitrifying
RT   bacterium, strain EbN1.";
RL   Arch. Microbiol. 183:27-36(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CR555306; CAI06845.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q5P766; -.
DR   SMR; Q5P766; -.
DR   STRING; 76114.ebA1330; -.
DR   PRIDE; Q5P766; -.
DR   EnsemblBacteria; CAI06845; CAI06845; ebA1330.
DR   KEGG; eba:ebA1330; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_4; -.
DR   OMA; LPIPKRM; -.
DR   Proteomes; UP000006552; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..696
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000197169"
SQ   SEQUENCE   696 AA;  75857 MW;  0A50326870C03818 CRC64;
     MMTTATVLVE LLTEELPPKA LPRLGETFAA KVFEGLKARD LVAEDRGFRC FAAPRRLAVT
     VPTVRAAAPS REVTEKIMPV QVALDAEGRP TPALLKKLQA KGIAPEAVAS FERRVDGKAE
     ALFYTHLEPG AALDDVLAAI VQEAVKALPI PKLMRWGAGD AQFVRPVHKL AMLHGARVVP
     GRVLDLDAGR VTMGHRFMSR GEIELATAEA YEPTLLAEGK VIPDFAERRS NIERQLVATA
     QEQGASLGEY ADLLDEVAAL VEHPTVYVGE FELEFLAVPQ ECLILTMRAN QKYFPLFDAA
     GKLLNRFLIV SNMRLADPSN IVAGNQRVVR PRLSDARFFF EQDRKHTLDS RLPRLAPVVY
     HNKLGSQLER VERLERLAGR IADRLHGDVA AASRAARLAK ADLVTDMVGE FPELQGIMGR
     YYALNDGEGE VVADAVQSHY QPRFAGDTLP AGNTACAVAL ADKLDTLVGF FGIGQLPTGD
     KDPFGLRRAA LGVLRILIET PLPLDLAALV GDAAEGFAPG LLTAADFESQ LLDFMFERLR
     NLLREAGHAV DVVDAVLALH PTRIDLVPAK LDAVRVFRGL PEAEALAAAN KRIVNILKKA
     EDELPEPDVA LLQEQAEKAL FHAVVEVAPL VHSHVANEDY TDALCALAGL RAAVDTFFDD
     VMVMVEEPLT RRNRLALLRQ LAGLMNQVAD LSRLSA