ID   SYGB_AZOSB              Reviewed;         695 AA.
AC   A1K3J8;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=azo0786;
OS   Azoarcus sp. (strain BH72).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Zoogloeaceae;
OC   Azoarcus.
OX   NCBI_TaxID=418699;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=BH72;
RX   PubMed=17057704; DOI=10.1038/nbt1243;
RA   Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J.,
RA   Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C.,
RA   Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J.,
RA   Weidner S., Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A.;
RT   "Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp.
RT   strain BH72.";
RL   Nat. Biotechnol. 24:1385-1391(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; AM406670; CAL93403.1; -; Genomic_DNA.
DR   RefSeq; WP_011764520.1; NC_008702.1.
DR   AlphaFoldDB; A1K3J8; -.
DR   SMR; A1K3J8; -.
DR   STRING; 62928.azo0786; -.
DR   PRIDE; A1K3J8; -.
DR   EnsemblBacteria; CAL93403; CAL93403; azo0786.
DR   KEGG; azo:azo0786; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_4; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000002588; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..695
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101264"
SQ   SEQUENCE   695 AA;  74661 MW;  5C2B5C7AC91AE0D2 CRC64;
     MTANTLLVEL LTEELPPKAL PRLGETFATK IAEGLKARGL AAADGAFRWF ASPRRIAITM
     AGVAAEAAAK EVTEKLMPVA VALDADGKPT PALLKKMEAK GIPASAVPAF ERRMDGKAEA
     LFHTAVVPGA RLAEVLAAIV HDAVKALPIP KVMRWGDGDA TFVRPVHKLV MLHGAEVVPG
     RVLDLDAGRS TRGHRFMSRG EIDIATADAY EPTLLAEGKV VPDFAERRAD IERQLLAEAA
     RLGAQLDDYT DLLDEVTALV EHPTVYVGEF EAEFLAVPQE CLILTMRANQ KYFPLFDAAG
     KLQNRFLIVS NMRMEDPVNI VTGNARVVRP RLSDARFFFE TDKKHKLDSR LPRLANVVYH
     NKLGSVLERV ERLEALAGKI AARLGSDEAA ARRAARLAKA DLVSEMVGEF PELQGIMGRY
     YALHDGEGAV VADAVQAHYQ PRFAGDALPA GNVAAAVALA DKLDALVGFF GIGMVPTGDK
     DPFALRRAAL GVLRILMEAP LPLDLAALVA DAAAGFKPGL LTADGFQAQL LDFMRERLKN
     LLRESGGEVA AIDAVLALAP TRIDLVPAKL AAVEAFRALP EAEALAAANK RIVNILKKAE
     GAPGEPDVAL LQEDAEKALF HRVIEIAPLV KSHVANEDYT DALCVLAGLR AEVDAFFDGV
     MVMAEEPLTR QNRLALLAQL AGLMNQVADI SRLSA