ID   SYGB_AZOVD              Reviewed;         685 AA.
AC   C1DFV1;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   26-MAY-2009, sequence version 1.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=Avin_00100;
OS   Azotobacter vinelandii (strain DJ / ATCC BAA-1303).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Azotobacter.
OX   NCBI_TaxID=322710;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DJ / ATCC BAA-1303;
RX   PubMed=19429624; DOI=10.1128/jb.00504-09;
RA   Setubal J.C., Dos Santos P., Goldman B.S., Ertesvaag H., Espin G.,
RA   Rubio L.M., Valla S., Almeida N.F., Balasubramanian D., Cromes L.,
RA   Curatti L., Du Z., Godsy E., Goodner B., Hellner-Burris K., Hernandez J.A.,
RA   Houmiel K., Imperial J., Kennedy C., Larson T.J., Latreille P., Ligon L.S.,
RA   Lu J., Maerk M., Miller N.M., Norton S., O'Carroll I.P., Paulsen I.,
RA   Raulfs E.C., Roemer R., Rosser J., Segura D., Slater S., Stricklin S.L.,
RA   Studholme D.J., Sun J., Viana C.J., Wallin E., Wang B., Wheeler C., Zhu H.,
RA   Dean D.R., Dixon R., Wood D.;
RT   "Genome sequence of Azotobacter vinelandii, an obligate aerobe specialized
RT   to support diverse anaerobic metabolic processes.";
RL   J. Bacteriol. 191:4534-4545(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP001157; ACO76278.1; -; Genomic_DNA.
DR   RefSeq; WP_012698706.1; NC_012560.1.
DR   AlphaFoldDB; C1DFV1; -.
DR   SMR; C1DFV1; -.
DR   STRING; 322710.Avin_00100; -.
DR   EnsemblBacteria; ACO76278; ACO76278; Avin_00100.
DR   KEGG; avn:Avin_00100; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_6; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000002424; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..685
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000204599"
SQ   SEQUENCE   685 AA;  75077 MW;  A0C51690BC6B3508 CRC64;
     MSAQDFLVEL GSEELPPKAL KSLATAFLAG IEKGLAAAGL EYRVARFYAA PRRLAVLVEH
     LVAQQPDRTL NLDGPPVAAA FDAEGNPTQA ALGFARKCGV ELHEIDSSGA KLRYSRHIPG
     QPASALLPAI VQASLDELPI PKRMRWGTRK EEFVRPSQWL VMLFGNQVVD CEILAQKAGR
     ESMGHRFHHP DAVRISQPSS YLEDLRSAYV LADFAERREL IAGRVADLAA QQQGSAVVPE
     DLLDEVTALV EWPVPLVCSF EERFLTVPQE ALISTMQDNQ KYFCLLDANG KLLPCFITVA
     NIESRDPAQI VAGNEKVVRP RLTDAEFFFN QDKRQPLESF NQRLASVVFQ AQLGSVLEKA
     ERVSRLAGFI AERIGGDPQH AIRAGLLSKA DLASEMVGEF PELQGIAGYH YALNAGEPED
     IARALDEQYM PRGAGAQLPS TLTGAAVAMA DKLDTLVGIF GIGMPPTGSK DPYALRRAAL
     GILRILIEKQ LDLNLEEASH FAVAQYGTKV KASGLAEQVS EFVFDRLRAR YEDEGIDVSS
     YLAVRALQPA SAYDLDLRVQ AVQAFRRLPE AEALAAVNKR VSNLLGKYEG KLPAAIEARY
     FDNASEFSLY SALQQAQHAV RPLAAERRYR EALERLASLR DPVDAYFEAV LVNAEDPAIR
     ANRHAMLAQL RGLFLCVADI SVLGQ