ID   SYGB_BRASB              Reviewed;         701 AA.
AC   A5EED4;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   12-JUN-2007, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=BBta_2357;
OS   Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium; unclassified Bradyrhizobium.
OX   NCBI_TaxID=288000;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=BTAi1 / ATCC BAA-1182;
RX   PubMed=17540897; DOI=10.1126/science.1139548;
RA   Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C.,
RA   Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L.,
RA   Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z.,
RA   Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E.,
RA   Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G.,
RA   Emerich D., Vermeglio A., Medigue C., Sadowsky M.;
RT   "Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia.";
RL   Science 316:1307-1312(2007).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000494; ABQ34528.1; -; Genomic_DNA.
DR   RefSeq; WP_012042556.1; NC_009485.1.
DR   AlphaFoldDB; A5EED4; -.
DR   SMR; A5EED4; -.
DR   STRING; 288000.BBta_2357; -.
DR   PRIDE; A5EED4; -.
DR   EnsemblBacteria; ABQ34528; ABQ34528; BBta_2357.
DR   KEGG; bbt:BBta_2357; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_1_5; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000000246; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..701
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101267"
SQ   SEQUENCE   701 AA;  76576 MW;  CDE741E3AD9089CA CRC64;
     MPDLLLELFS EEIPARMQAK AAEDLRRMVT DKLVAEGLVY EGAKAFATPR RLALTVHGIP
     ARQADLKEER KGPRVGGPDA AIQGFLKATG LSSLEEATIQ RDPKKGDFYV ALIEKPGRAT
     LDVLAEMLPV IVRTFPWPKS MRWGKRSEKP GALNWVRPLH AITATFGLET EEPDVVSFSV
     DGIEAGQTTY GHRFMAPAAI SVRRFEDYEA KLLDAKVVLD PQRRKDTIVT DAKQLAFAQG
     YELVEDPVLL DEVSGLVEWP VVLMGSFDPE YLKVPAEVIR ATIRNNQKCF VVRDPKTGGL
     APKFILTANI EATDGGKTII AGNERVIRAR LSDAKFFYET DLKTRLEDRL PKFEQIVFHE
     KLGTQAARIA RIEKLAAEIA PLVGADAAKT ARAAKLAKAD LLTEVVGEFP EVQGLMGKYY
     ALAQGEDVSV AAACEEHYKP QGPGDRVPTD PVSVAVALAD KLDTLVGFWA IDEKPTGSKD
     PYALRRAALG VIRLITENSL RLSLLQVAGS ALAGLHVKSE LDAGKLSADL LSFFADRLKV
     QLREQGARHD LVDAVFALGG QDDLLMVVRR VEALGKFLET DDGKNLLAGT KRASNILAIE
     EKKDKRKFDG APDAALYRLD EEKALAKAIG EVGSEAGTAV AKEDFAAAMH AMAKLRPAVD
     AFFDKVKVND DDAAIRENRL KLLNEIRNAT RAVADFSKIE G