ID   SYGB_CERS1              Reviewed;         697 AA.
AC   A3PH07;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2007, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   OrderedLocusNames=Rsph17029_0507;
OS   Cereibacter sphaeroides (strain ATCC 17029 / ATH 2.4.9) (Rhodobacter
OS   sphaeroides).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Rhodobacteraceae; Cereibacter.
OX   NCBI_TaxID=349101;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 17029 / ATH 2.4.9;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Richardson P.,
RA   Mackenzie C., Choudhary M., Donohue T.J., Kaplan S.;
RT   "Complete sequence of chromosome 1 of Rhodobacter sphaeroides ATCC 17029.";
RL   Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000577; ABN75623.1; -; Genomic_DNA.
DR   RefSeq; WP_011840400.1; NC_009049.1.
DR   AlphaFoldDB; A3PH07; -.
DR   SMR; A3PH07; -.
DR   EnsemblBacteria; ABN75623; ABN75623; Rsph17029_0507.
DR   GeneID; 57469200; -.
DR   KEGG; rsh:Rsph17029_0507; -.
DR   HOGENOM; CLU_007220_2_1_5; -.
DR   OMA; LPIPKRM; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..697
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101325"
SQ   SEQUENCE   697 AA;  75665 MW;  87CB19DA4CADB376 CRC64;
     MPDLLIELFS EEIPARMQAR AREDLKKLVT DGLVEAGLTY ASAGAFSTPR RLVLSVEGLS
     AESPTLREER KGPKADAPAA AIEGFLRSTG LTRDRLETRE DKKGAVLFAV VEKPGRPAPE
     IVAEVLERTI RTFPWPKSMR WGTGSLRWVR PLQSILCLLS DEGGAEVVPM TLDGLTAGNS
     TEGHRFMAPA RFAVSGFEDY RAKLGRAFVM LDASEREQAI WHEATTQAFA QGLEVVPDAA
     LLSEVAGLVE WPVVLMGAIG EDFLGLPPEV LQTSMREHQK FFSVTNPATG RIEKFVTVAN
     RETADHGETI LKGNGKVLSA RLSDARFFWE NDLRTVKTAG LEGMAEGLKQ VTFHNRLGSQ
     ADRIARIEAL AREIAPLVGA SPDLAAEAAR VAKADLQSAM VGEFPELQGT MGSYYARAAG
     LPEAVAQACK AHYQPLGPSD AVPTDPVSVA VALADKIDTL AGFWRIGEKP TGSKDPFALR
     RAALGVIRLL LTNNSRAGLM GIMLPAMNRH LEPFDTSDFT PYERELLSFF HDRLKVHLRE
     QGIRHDVIDA CLAMPGNDDL TLLVKRAEAL SAFLKTDDGT NLLQGFKRAN NILTQAEAKD
     GVEYSFGADP KFAETDAERA LFAALETAEA AIGPALQAED FAAAMSAMAA LRAPIDAFFE
     TVQVNADNAV LRRNRLNMLH SIRATCARVA DLTRIEG