ID   SYGB_CERS4              Reviewed;         697 AA.
AC   Q3J5C7;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   25-MAY-2022, entry version 105.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=RHOS4_04390;
GN   ORFNames=RSP_1858;
OS   Cereibacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / CCUG
OS   31486 / LMG 2827 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.) (Rhodobacter
OS   sphaeroides).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Rhodobacteraceae; Cereibacter.
OX   NCBI_TaxID=272943;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 17023 / DSM 158 / JCM 6121 / CCUG 31486 / LMG 2827 / NBRC 12203
RC   / NCIMB 8253 / ATH 2.4.1.;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA   Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C.,
RA   Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S.;
RT   "Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1.";
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000143; ABA78007.1; -; Genomic_DNA.
DR   RefSeq; WP_011337035.1; NZ_CP030271.1.
DR   RefSeq; YP_351908.1; NC_007493.2.
DR   AlphaFoldDB; Q3J5C7; -.
DR   SMR; Q3J5C7; -.
DR   STRING; 272943.RSP_1858; -.
DR   EnsemblBacteria; ABA78007; ABA78007; RSP_1858.
DR   KEGG; rsp:RSP_1858; -.
DR   PATRIC; fig|272943.9.peg.746; -.
DR   eggNOG; COG0751; Bacteria.
DR   OMA; LPIPKRM; -.
DR   PhylomeDB; Q3J5C7; -.
DR   Proteomes; UP000002703; Chromosome 1.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..697
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101326"
SQ   SEQUENCE   697 AA;  75591 MW;  3132E3346CA0656A CRC64;
     MPDLLIELFS EEIPARMQAR AREDLKKLVT DGLVEAGLTY ASAGAFSTPR RLVLSVEGLS
     AESPTLREER KGPKADAPAA AIEGFLRSTG LTRDQLETRE DKKGAVLFAV VEKPGRPAPE
     IVAEVLERTI RTFPWPKSMR WGTGSLRWVR PLQSILCLLS DEAGAEVVPM TLDGLTAGNS
     TEGHRFMAPA RFAVSGFEDY RAKLARAFVM LDASEREQAI WHEATTQAFA QGLEVVPDAA
     LLSEVAGLVE WPVVLMGAIG EDFLGLPPEV LQTSMREHQK FFSVTNPATG RIEKFVTVAN
     RETADHGETI LKGNGKVLSA RLSDARFFWE NDLRTVKTAG LEGMAEGLKQ VTFHNRLGSQ
     AARIARIEAL AREIAPLVGA SPDLAAEAAR VAKADLQSAM VGEFPELQGT MGSYYARAAG
     LPEAVAQACK AHYQPLGPSD AVPTDPVSVA VALADKIDTL AGFWRIGEKP TGSKDPFALR
     RAALGVIRLL LTNNSRAGLM GIMLPAMNRH LEPFDTSDFT PYERDLLAFF HDRLKVHLRE
     QGIRHDVIDA CLAMPGNDDL TLLVKRAEAL SAFLKTDDGT NLLQGFKRAN NILTQAEAKD
     GVEYSFGADP KFAETDAERA LFAALETAEA AIGPALQAED FAAAMSAMAA LRAPIDAFFE
     TVQVNADNAV LRRNRLNMLH SIRATCARVA DLTRIEG