ID   SYGB_DESAL              Reviewed;         690 AA.
AC   B8FKD8;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=Dalk_0043;
OS   Desulfatibacillum aliphaticivorans.
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Desulfobacterales;
OC   Desulfobacteraceae; Desulfatibacillum.
OX   NCBI_TaxID=218208;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AK-01;
RX   PubMed=21651686; DOI=10.1111/j.1462-2920.2011.02516.x;
RA   Callaghan A.V., Morris B.E., Pereira I.A., McInerney M.J., Austin R.N.,
RA   Groves J.T., Kukor J.J., Suflita J.M., Young L.Y., Zylstra G.J., Wawrik B.;
RT   "The genome sequence of Desulfatibacillum alkenivorans AK-01: a blueprint
RT   for anaerobic alkane oxidation.";
RL   Environ. Microbiol. 14:101-113(2012).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP001322; ACL01753.1; -; Genomic_DNA.
DR   RefSeq; WP_012609193.1; NC_011768.1.
DR   AlphaFoldDB; B8FKD8; -.
DR   SMR; B8FKD8; -.
DR   EnsemblBacteria; ACL01753; ACL01753; Dalk_0043.
DR   KEGG; dal:Dalk_0043; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_7; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000000739; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..690
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000204600"
SQ   SEQUENCE   690 AA;  75448 MW;  CA402966AC7FF184 CRC64;
     MQPLLLEIGS EEIPAGYIVP ALEALAQALD AKLESARIAH SKPKVYGTPR RLAVILDEVA
     EKQESVTTEM VGPPKSVAFD GEGKPKVPAV KFAEKAGVAV EELTFQETEK GVYLCAKIQD
     EGKETLGILQ EMLPEIISHI PFPKSMRWAA LPGTFARPVF SILALLGDQV IPFEWNGVTT
     GRQTRGHYFM APEAIDIQTP SEYVNALEKA KVIADIPTRR KMVKEGVDAI AKELGGDAIE
     DEELVDIVAN LVEFPAPVGG KFETGFLEVP DKVLITAMRE HQKYFAIQDK DGKLMPCFVA
     VNNTQCKDPQ LVATGHERVL RARLSDAKFF WDVDKKQSME DWVKRLDRVL FQKKLGSVGE
     KVARVEEMAK FLAAAPEING DPDKAQKAAH FCKADLVSGL VIEFTKLQGV MGKAYASLAG
     MDAETASALE EHYLPAYSGG PLPRTKTGDA VAMADKMDSL CGCFAVGLIP SGNRDPYALR
     RQGIGVIRIL QEKGYSLSLS AIVDKGLTLV KDKADQNLAE TRDKIISFLA DRMAHMLAEQ
     GFSKDVIQAA VAISCDDIPY LWKRVAAVEK LKTLPDYEAL AQTFKRVANI IKQAAEKGTL
     SDQEVNPALF EKDCEKDLLE AFTAMEAKVS GLGVDEALLE VAKLRPAVDA FFDDVMVMAE
     DMKVRENRLA LLAGIAGLFG RFADFSRISA