ID   SYGB_DINSH              Reviewed;         720 AA.
AC   A8LS45;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   04-DEC-2007, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=Dshi_2402;
OS   Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Roseobacteraceae; Dinoroseobacter.
OX   NCBI_TaxID=398580;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 16493 / NCIMB 14021 / DFL 12;
RX   PubMed=19741735; DOI=10.1038/ismej.2009.94;
RA   Wagner-Dobler I., Ballhausen B., Berger M., Brinkhoff T., Buchholz I.,
RA   Bunk B., Cypionka H., Daniel R., Drepper T., Gerdts G., Hahnke S., Han C.,
RA   Jahn D., Kalhoefer D., Kiss H., Klenk H.P., Kyrpides N., Liebl W.,
RA   Liesegang H., Meincke L., Pati A., Petersen J., Piekarski T.,
RA   Pommerenke C., Pradella S., Pukall R., Rabus R., Stackebrandt E., Thole S.,
RA   Thompson L., Tielen P., Tomasch J., von Jan M., Wanphrut N., Wichels A.,
RA   Zech H., Simon M.;
RT   "The complete genome sequence of the algal symbiont Dinoroseobacter shibae:
RT   a hitchhiker's guide to life in the sea.";
RL   ISME J. 4:61-77(2010).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000830; ABV94138.1; -; Genomic_DNA.
DR   RefSeq; WP_012179069.1; NC_009952.1.
DR   AlphaFoldDB; A8LS45; -.
DR   SMR; A8LS45; -.
DR   STRING; 398580.Dshi_2402; -.
DR   EnsemblBacteria; ABV94138; ABV94138; Dshi_2402.
DR   KEGG; dsh:Dshi_2402; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_1_5; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000006833; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   SMART; SM00836; DALR_1; 1.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..720
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101276"
SQ   SEQUENCE   720 AA;  79130 MW;  79ED61C0A73470FC CRC64;
     MPELLIELFS EEIPARMQAR AAQDLQKLVT NGLVEAGITY AGARAYATPR RLTLVVSDML
     AASAPVREER KGPKADAPEK AIEGFLRSTG LGREQLETRE TPKGDVLFAV IEKPGRPAAE
     IVAEVLETTI RNFPWPKSMR WGSSSLRWVR PLHRILCLIR DEEGAEIVPL TVDGITAGDV
     TEGHRFLAPG EIKVHGFEDY ETKLRRAKVI LDAEERAEHI WADATNQAFA QGLEVVEDRR
     LLSEVAGLVE FPVPLMGEID ADFLDLPPEV LQTSMKEHQK FFSVRDLKSG KIVRFVTVAN
     TETTDHGATI LAGNRKVLSA RLADAKFFWE NDLRIARAGM QPWREALENV TFHNKLGSQA
     ARIARIADLA ATLAPQVGAD PDAARTAAEL AKADLSSEMV FEFPELQGLM GRYYAEAAGH
     PAEIAAVAQD HYQPLGPSDA VPTAPLSIAV ALADKLDTLT GFWAIDEKPT GSKDPFALRR
     AALGVIRIVL ENGLRLPLIN SVKHHNSENT SRPLFSALVA HAKTDQERAD GSKSMSDWKA
     LVARSQDDLL SFFHDRLKVF LRDEGIRHDI IDACLAMEGN DDLLLLVNRA RALSETLKTE
     DGENLLQGFK RANNILTQAE EADGVEYSYG ADPKFAETDE ERALFAALDT AEAVIDPALE
     AEDFAAATAA MASLRAPLDA FFEAVQVNAE NQILRRNRLN LLSRIRATCL KLADLTRIEG