ID   SYGB_ENTFA              Reviewed;         694 AA.
AC   Q831U3;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=EF_2406;
OS   Enterococcus faecalis (strain ATCC 700802 / V583).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
OC   Enterococcus.
OX   NCBI_TaxID=226185;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700802 / V583;
RX   PubMed=12663927; DOI=10.1126/science.1080613;
RA   Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R.,
RA   Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H.,
RA   Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C.,
RA   DeBoy R.T., Durkin S.A., Kolonay J.F., Madupu R., Nelson W.C.,
RA   Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M.,
RA   Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M.;
RT   "Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus
RT   faecalis.";
RL   Science 299:2071-2074(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; AE016830; AAO82125.1; -; Genomic_DNA.
DR   RefSeq; NP_816055.1; NC_004668.1.
DR   RefSeq; WP_002359749.1; NZ_KE136528.1.
DR   AlphaFoldDB; Q831U3; -.
DR   SMR; Q831U3; -.
DR   STRING; 226185.EF_2406; -.
DR   EnsemblBacteria; AAO82125; AAO82125; EF_2406.
DR   KEGG; efa:EF2406; -.
DR   PATRIC; fig|226185.45.peg.1137; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_9; -.
DR   OMA; LPIPKRM; -.
DR   Proteomes; UP000001415; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..694
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000006358"
SQ   SEQUENCE   694 AA;  78560 MW;  6094D98E1C3AEC69 CRC64;
     MAKDLLLEIG LEEMPAHVVT PSRIQLEEKV IKFLDEHHLD YETVQSFATP RRLAVKVTAI
     PEKQADVEEE VKGPAKKIAL DAEGNWSKAA QGFVRGQGVT TEDIVFKELN GVEYVYVTKF
     TKGQSAKEVL TKLNDVITSL TFPVTMHWAN YDFEYIRPIH WIVALLDDEV IPFKVLDVTT
     GQTSRGHRFL GDDVTFQHAN EYEAKLKEQF VVVQPNERKQ MIVDQANALA AEKNWQLALD
     EELLEEVTNL VEYPTAFVGS FDEKYLSVPD EVLVTSMKEH QRYFDVRNDQ GLLMPHFIAV
     RNGDNVHLEN VIKGNEKVLI ARLEDAEFFY NEDKKLTIEA CVEKLKNVTF HEKIGSIYEK
     MQRVALIAQI IGRKVGLSED ELEDLKRASE IYKFDLVTNM VGEFPELQGI MGEKYALLQG
     EKPAVATAIR EHYLPTSSEG ELPETAIGAV LALADKLDSV FSFFSVGMIP TGSNDPYALR
     RQTYGVIRII EDKGWTFPLV QLQTEVDEAV NQDVEKYGVL LNEGQAEVVE FVKARLRQLL
     MTKNVRHDII DAVVSAEQAD LSKLFASANI LKSRFEDQDF KPSMEALTRV INLAKKGQEL
     LGDTEEGIDP SLFENKAEKE LYQAVNDLSE AFATRTIAEN YEALVNLRPL IDAYFNETMV
     MVEDEKVKQN RLKQLMQIAK MALSIASLDL LIVK