ID   SYGB_FUSNN              Reviewed;         686 AA.
AC   Q8RH44;
DT   08-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=FN0070;
OS   Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / DSM 15643 /
OS   BCRC 10681 / CIP 101130 / JCM 8532 / KCTC 2640 / LMG 13131 / VPI 4355).
OC   Bacteria; Fusobacteria; Fusobacteriales; Fusobacteriaceae; Fusobacterium.
OX   NCBI_TaxID=190304;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25586 / DSM 15643 / BCRC 10681 / CIP 101130 / JCM 8532 / KCTC
RC   2640 / LMG 13131 / VPI 4355;
RX   PubMed=11889109; DOI=10.1128/jb.184.7.2005-2018.2002;
RA   Kapatral V., Anderson I., Ivanova N., Reznik G., Los T., Lykidis A.,
RA   Bhattacharyya A., Bartman A., Gardner W., Grechkin G., Zhu L., Vasieva O.,
RA   Chu L., Kogan Y., Chaga O., Goltsman E., Bernal A., Larsen N., D'Souza M.,
RA   Walunas T., Pusch G., Haselkorn R., Fonstein M., Kyrpides N.C.,
RA   Overbeek R.;
RT   "Genome sequence and analysis of the oral bacterium Fusobacterium nucleatum
RT   strain ATCC 25586.";
RL   J. Bacteriol. 184:2005-2018(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; AE009951; AAL94283.1; -; Genomic_DNA.
DR   RefSeq; NP_602984.1; NC_003454.1.
DR   AlphaFoldDB; Q8RH44; -.
DR   SMR; Q8RH44; -.
DR   STRING; 190304.FN0070; -.
DR   EnsemblBacteria; AAL94283; AAL94283; FN0070.
DR   KEGG; fnu:FN0070; -.
DR   PATRIC; fig|190304.8.peg.662; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_0; -.
DR   InParanoid; Q8RH44; -.
DR   OMA; LPIPKRM; -.
DR   BioCyc; FNUC190304:G1FZS-683-MON; -.
DR   Proteomes; UP000002521; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..686
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_0000072902"
SQ   SEQUENCE   686 AA;  78744 MW;  963D02A304466AB6 CRC64;
     MELLFEIGME EIPARFLNQA LEDLKSNFEK KLKNNRIKFD GVRTYGTPRR LVLVADEVAG
     MQEDLDELSI GPSRERAYKD GALTKAGEGF LKAHRIEEEQ IEIIKNDKGE YIAFKRFLKG
     KPTEAILPEI LKALVLEETF QKSMRWSTKT IRFARPIEWF LALYGGKVID FEIEGIKSSN
     KSKGHRFFGK EFEVSSVENY LKKIRENNVI IDISERRKMI EEMINNSLLE DEKADVDKAL
     LDEVTNLVEH PFAIVGTFSE EFLEVPQEVL IISMKVHQRY FPILNKKGKL LPKFIVIRNG
     IDFSENVKKG NEKVLSARLA DARFFYYEDL KIPLDNNVEK LKTVVFQKDL GTIYNKVKRC
     EKIAEFLVGK LKYNYMKEDI LRTVKLAKAD LVSNMINEKE FTKLQGFMGE NYALKAGEEI
     GVALGIKEHY YPRFQGDLLP SGIEGIIAGI SDRIDTLVGC FGVGVIPTGS KDPFALRRTA
     LGIVNIIINA NLDISLKDLV KVSLDALEED KVLKTDRAKV ETDVLDFLKQ RIINVFTDMK
     YRKDVILAVL DKDSDNITTA LEIVKVITEK LSKDKMQALL QVVKRVSNIM KGNKDVTIKE
     KLFKTDIEKT LYAESKKVES EVEQAIKEKE YADYFEKLFT LVPTIDKYFE TVIVMDEDKN
     VRDNRIGQLT YIMNLFEKIA YLNKLD