ID   SYGB_GEODF              Reviewed;         687 AA.
AC   B9M5V4;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAR-2009, sequence version 1.
DT   25-MAY-2022, entry version 63.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=Geob_1577;
OS   Geotalea daltonii (strain DSM 22248 / JCM 15807 / FRC-32) (Geobacter
OS   daltonii).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Desulfuromonadales;
OC   Geobacteraceae; Geotalea.
OX   NCBI_TaxID=316067;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 22248 / JCM 15807 / FRC-32;
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C.,
RA   Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.,
RA   Kostka J., Richardson P.;
RT   "Complete sequence of Geobacter sp. FRC-32.";
RL   Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP001390; ACM19935.1; -; Genomic_DNA.
DR   RefSeq; WP_012646664.1; NC_011979.1.
DR   AlphaFoldDB; B9M5V4; -.
DR   SMR; B9M5V4; -.
DR   STRING; 316067.Geob_1577; -.
DR   PRIDE; B9M5V4; -.
DR   EnsemblBacteria; ACM19935; ACM19935; Geob_1577.
DR   KEGG; geo:Geob_1577; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_7; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000007721; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..687
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000197201"
SQ   SEQUENCE   687 AA;  75505 MW;  CE9FFDA1B3ED00EE CRC64;
     MAKELFLEIG TEEIPAGFLP KAMADMEGLI RKELESARIG FGEVKTMATP RRLALVVKNV
     SAQQTDAEIT TMGPAKKVAF NDDGTTTKAG EGFARGQGVD ASALSIIATE KGEYVAVTKK
     EIGVATAGLL AEILPRLINN IPFKKSMRWG DQEVRFARPM HWIVALFDGT VIPFAFGNVQ
     SGSMSRGHRF MANTSFPVRD FGHYLEECER HFVIPDPAKR KEIISREIER VAKAAGGNVL
     PDEGLLEQVT YLVEYPSAVH GTFSAEFLAV PKEVLITSMR EHQRYFSLVD DKGKLLPGFI
     TINNTLTEDP SVVVKGNEMV LRARLSDARF FFEEDKKVPL EKRVEALKSV LYQAKLGTSY
     EKMERFRTLA EGLAEQLQPP LKAKVFQAAT LCKADLVTGM VGEFPEVQGI MGREYALLQG
     VDAEVARAIA EHYLPNQAGG ELPASDTGAF VSIADKVDTI CGCFSVGLIP SGSADPYALR
     RAALGIINII LAKGYNLPLI PLVTKAIGQL EGRLTRKKEE VLADVLDFFK GRFINLLTDR
     FPADVVEAVV AVSFDNLVEA TAKIEALAQF KKRDDFEPLA VAFKRVCNIV KEQVTVPVDD
     KLFQDAAEGT LFQSFKSASS MVEEKVVQRE YLAALTQIAS LKGAVDDFFD KVMVMAEDEG
     VRNNRLALLQ EIKSLFRDIA DFGKLTA