ID   SYGB_GEOSM              Reviewed;         687 AA.
AC   C6E0B4;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-SEP-2009, sequence version 1.
DT   25-MAY-2022, entry version 61.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=GM21_0656;
OS   Geobacter sp. (strain M21).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Desulfuromonadales;
OC   Geobacteraceae; Geobacter; unclassified Geobacter.
OX   NCBI_TaxID=443144;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=M21;
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C.,
RA   Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.,
RA   Lovley D.;
RT   "Complete sequence of Geobacter sp. M21.";
RL   Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP001661; ACT16730.1; -; Genomic_DNA.
DR   RefSeq; WP_012774350.1; NC_012918.1.
DR   AlphaFoldDB; C6E0B4; -.
DR   SMR; C6E0B4; -.
DR   STRING; 443144.GM21_0656; -.
DR   EnsemblBacteria; ACT16730; ACT16730; GM21_0656.
DR   KEGG; gem:GM21_0656; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_7; -.
DR   OMA; LPIPKRM; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..687
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000204605"
SQ   SEQUENCE   687 AA;  75808 MW;  ACBF54EF43EDA723 CRC64;
     MAKDLFLEIG CEEIPAGFVP KAMADMELLI KKEFDSARIE YGEIVTLGTP RRLVLAVKGV
     AERQPDAELT AMGPAKSHAY DADGNPTKAA QGFARGQGID VSQLKLVTTE KGEYLAAVKS
     EIGRETAELL PEMLPRLIGN IPFKKSMRWA DFDVRFARPI HWIAALFDGK VVPFSFGNID
     SGSASRGHRF MANTPFPVRD LAHYLEECER HFVIPDPNKR KEIIRAEIER VAKQAKGNVL
     PDEALLEQVS YLVEYPSAVH GTFSPDFLVV PREVLITSMR EHQRYFSLVD DEGKLLPGFI
     TINNTITEDP QVVVKGNERV LRARLSDARF FFDEDHKVRL ETRVESLKSV VYQAKLGTSY
     EKMERFRELG KRLAQRLNPS VIKQVERAAT LCKADLVSGM VGEFPEVQGI MGREYALHDG
     EEPAVANAIA EHYLPTQAGG ELPASDIGAF VSLADKMDTI CGCFCVGLIP TGSADPYALR
     RSALGIINII LEKGYREPLS EFVKASLDLL AAKATRPLAE VQKDVLDFFR GRFVNLMADR
     FPSDAVEAVA SVSFDDLVEA AAKIEALAGF RNRDDFGPLA VAFKRVCNIV KDGVDTPVSA
     ELFQDAAEGE LHQALTQVSG KVADALKKAD YLAALTEIAT LKPAVDLFFE KVMVMADDER
     VRQNRLALLT GIARLFASLA DFSRLSP