ID   SYGB_GLOC7              Reviewed;         717 AA.
AC   B7KD52;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   03-AUG-2022, entry version 64.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   OrderedLocusNames=PCC7424_4816;
OS   Gloeothece citriformis (strain PCC 7424) (Cyanothece sp. (strain PCC
OS   7424)).
OC   Bacteria; Cyanobacteria; Oscillatoriophycideae; Chroococcales;
OC   Aphanothecaceae; Gloeothece; Gloeothece citriformis.
OX   NCBI_TaxID=65393;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 7424;
RX   PubMed=21972240; DOI=10.1128/mbio.00214-11;
RA   Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H.,
RA   Sherman L.A., Pakrasi H.B.;
RT   "Novel metabolic attributes of the genus Cyanothece, comprising a group of
RT   unicellular nitrogen-fixing Cyanobacteria.";
RL   MBio 2:E214-E214(2011).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP001291; ACK73173.1; -; Genomic_DNA.
DR   RefSeq; WP_015956755.1; NC_011729.1.
DR   AlphaFoldDB; B7KD52; -.
DR   SMR; B7KD52; -.
DR   STRING; 65393.PCC7424_4816; -.
DR   EnsemblBacteria; ACK73173; ACK73173; PCC7424_4816.
DR   KEGG; cyc:PCC7424_4816; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_3; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000002384; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..717
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000197173"
SQ   SEQUENCE   717 AA;  80555 MW;  248C45A44A1EF7FC CRC64;
     MPSFLLEVGT EELPANFVDE AIAQWQSRIP SSLEEQQLTP EGIEFYGTPR RLAVLIKGLP
     AKQSDRIEEV KGPAANVAFK SGKPTKALEG FVRKQGVTPD EVEIRDTDKG EFVFVQKNIT
     GRNTTEILQE LVPQWITQLE GRRFMRWGDG DLRFPRPIRW LVTLWDQDIL PLELVNGATQ
     VKSDRLTYGH RILAPESVSI PEASAYQKSL QQAYVEVSPL QRRQTIEQQI EKVAKQLKGV
     AVIPPDLLDE VVNLVEYPSA VAGNFEPDFL NLPTEVITTV MVTHQRYFPV KATGKTNKNN
     TLLPYFITIS NGDPNKGEII AAGNERVIRA RLADAQFFYK ADCDEPLESY LPQLETVTFQ
     EQLGTIRDKV DRIMEISQLI ADQLDLDSGE RSEIESTAML CKADLVTQMV YEFPELQGVM
     GQKYALASGE SPNVALGIFE HYLPRGADDI MPESLTGQVV GLADRIDTIV SIFGLGMIPT
     GSGDPFALRR AATGIIKVTW YAELPIDILD LLEQSSQDFV TAHPDKTSPI ESLKSFFIQR
     LSTLLQDDLH IDYDLVNAVL GENDPEYTER ALRDLLDVRD RAQFLQSIRN NQKIDEIYET
     VNRSARLAVK GELDTQELDP EKVIRPALFE KSSEEHFYKV LIDLVPTTLT AQRERNYQLL
     VDALGKIAPT VSNFFDGEDS VLVMDENLEV RENRLNLLGL LRNHARVLAD FGAIVKS