ID   SYGB_HAEI8              Reviewed;         722 AA.
AC   Q4QLY5;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   25-MAY-2022, entry version 107.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=NTHI1093;
OS   Haemophilus influenzae (strain 86-028NP).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=281310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=86-028NP;
RX   PubMed=15968074; DOI=10.1128/jb.187.13.4627-4636.2005;
RA   Harrison A., Dyer D.W., Gillaspy A., Ray W.C., Mungur R., Carson M.B.,
RA   Zhong H., Gipson J., Gipson M., Johnson L.S., Lewis L., Bakaletz L.O.,
RA   Munson R.S. Jr.;
RT   "Genomic sequence of an otitis media isolate of nontypeable Haemophilus
RT   influenzae: comparative study with H. influenzae serotype d, strain KW20.";
RL   J. Bacteriol. 187:4627-4636(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000057; AAX87962.1; -; Genomic_DNA.
DR   RefSeq; WP_011272293.1; NC_007146.2.
DR   AlphaFoldDB; Q4QLY5; -.
DR   SMR; Q4QLY5; -.
DR   PRIDE; Q4QLY5; -.
DR   EnsemblBacteria; AAX87962; AAX87962; NTHI1093.
DR   KEGG; hit:NTHI1093; -.
DR   HOGENOM; CLU_007220_2_2_6; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000002525; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 2.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   SMART; SM00836; DALR_1; 1.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..722
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000006363"
SQ   SEQUENCE   722 AA;  79688 MW;  AED5FA2017E84441 CRC64;
     MTTQNFLVEI GTEELPPKAL KTLATSFADN VEAELNQAGL SFDKIEWFAA PRRLAVKVLN
     LATQQPSKEI EKRGPAVSAA FDAEGKPTKA AEGWARGCGI TVEQAERIAT DKGEWLVHRT
     KIKGQPTKNL LNDIVANALA KLPIPKPMRW ADKTVQFIRP VHTVTMLLGD ELIEGEILGV
     ASARTIRGHR FLGEKEFDIQ HADQYPQLLR DKGSVVADFN ERKAEILAKS QAKATALGGV
     ADIEESLLEE VTSLVEYPNV LAAKFEERFL AVPAEALVYT MKGDQKYFPI YDKDGRLLPH
     FIFVSNINPE DPTAIIEGNE KVVRPRLTDA EFFFKTDLKQ KLVDRLPRLE TVLFQQQLGT
     LKDKTDRIEQ LAGEIAKQIG ADEAKAKRAG LLSKCDLMTN MVFEFTDTQG VMGMHYARHD
     GEDEEVAVAL NEQYMPRFAG DELPKSLVAS AVALADKFDT LTGIFGIGQA PKGSADPFAL
     RRAALGALRI IVEKNLPLDL NDIISKAFDL YKELDNERLR NAPIAKTRGG FSEYPEGYVS
     FFTRGDDLVP KQKILDEVVD FMLGRFRAWY QDEGIAVDVI QAVLARRPTR PADFDARVRA
     VSHFRTLDSA EALAAANKRV SNILAKAGAA IGEINLTACV EPAEKALAEA VLALRTEVQP
     LIAQGDYTAV LDKLANLRAP VDSFFDNVMV NAEDPALRQN RLAILNTLQG LFLQVADISV
     LQ