ID   SYGB_HAEIE              Reviewed;         688 AA.
AC   A5UDE3;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   OrderedLocusNames=CGSHiEE_07355;
OS   Haemophilus influenzae (strain PittEE).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=374930;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PittEE;
RX   PubMed=17550610; DOI=10.1186/gb-2007-8-6-r103;
RA   Hogg J.S., Hu F.Z., Janto B., Boissy R., Hayes J., Keefe R., Post J.C.,
RA   Ehrlich G.D.;
RT   "Characterization and modeling of the Haemophilus influenzae core and
RT   supragenomes based on the complete genomic sequences of Rd and 12 clinical
RT   nontypeable strains.";
RL   Genome Biol. 8:R103.1-R103.18(2007).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000671; ABQ98794.1; -; Genomic_DNA.
DR   RefSeq; WP_005686190.1; NC_009566.1.
DR   AlphaFoldDB; A5UDE3; -.
DR   SMR; A5UDE3; -.
DR   KEGG; hip:CGSHiEE_07355; -.
DR   HOGENOM; CLU_007220_2_2_6; -.
DR   OMA; LPIPKRM; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   SMART; SM00836; DALR_1; 1.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..688
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000006364"
SQ   SEQUENCE   688 AA;  75616 MW;  9C38C97948978128 CRC64;
     MTTQNFLVEI GTEELPPKAL KTLATSFADN VEAELIQAGL SFDKIEWFAA PRRLAVKVLN
     LATQQPSKEI EKRGPAVSAA FDAEGKPTKA AEGWARGCGI TVEQAERIAT DKGEWLVHCA
     KIEGQPTKNL LNDIVANALA KLPIPKPMRW ADKTVQFIRP VHTVTMLLGD ELIEGEILGV
     ASARTIRGHR FLGEKEFEIQ HADQYPQLLR EKGSVVADFN ERKAEILAKS QAKATALGGV
     ADIEESLLEE VTSLVEYPNV LAAKFEERFL AVPAEALVYT MKGDQKYFPI YDKDGKLLPH
     FIFVSNINPE DPTAIIEGNE KVVRPRLTDA EFFFKTDLKQ KLVDRLPRLE TVLFQQQLGT
     LKDKTDRIEQ LAGEIAKQIG ADEAKAKRAG LLSKCDLMTN MVFEFTDTQG VMGMHYARHD
     GEDEEVAVAL NEQYMPRFAG DELPKSLVAS AVALADKFDT LTGIFGIGQA PKGSADPFAL
     RRAALGALRI IVEKNLPLDL EDLVKKSAAL FSDKLTNKNV VADVVDFMLG RFRAWYQDEG
     IAVDVIQAVL ARRPTRPADF DARVRAVSHF RTLDSAEALA AANKRVSNIL AKADAAIGEI
     NLTACVEPAE KALAEAVLVL RTEVQPLIAQ GDYTAVLDKL ANLRAPVDSF FDNVMVNAED
     PALRQNRLAI LNTLQGLFLQ VADISVLQ