ID   SYGB_HAHCH              Reviewed;         692 AA.
AC   Q2SQY0;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   25-MAY-2022, entry version 95.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=HCH_00021;
OS   Hahella chejuensis (strain KCTC 2396).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales;
OC   Hahellaceae; Hahella.
OX   NCBI_TaxID=349521;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KCTC 2396;
RX   PubMed=16352867; DOI=10.1093/nar/gki1016;
RA   Jeong H., Yim J.H., Lee C., Choi S.-H., Park Y.K., Yoon S.H., Hur C.-G.,
RA   Kang H.-Y., Kim D., Lee H.H., Park K.H., Park S.-H., Park H.-S., Lee H.K.,
RA   Oh T.K., Kim J.F.;
RT   "Genomic blueprint of Hahella chejuensis, a marine microbe producing an
RT   algicidal agent.";
RL   Nucleic Acids Res. 33:7066-7073(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000155; ABC26944.1; -; Genomic_DNA.
DR   RefSeq; WP_011394021.1; NC_007645.1.
DR   AlphaFoldDB; Q2SQY0; -.
DR   SMR; Q2SQY0; -.
DR   STRING; 349521.HCH_00021; -.
DR   PRIDE; Q2SQY0; -.
DR   EnsemblBacteria; ABC26944; ABC26944; HCH_00021.
DR   KEGG; hch:HCH_00021; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_6; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000000238; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   SMART; SM00836; DALR_1; 1.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..692
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000006367"
SQ   SEQUENCE   692 AA;  77189 MW;  BD1F2472A7E1D73C CRC64;
     MNHQDFLVEI GAEELPPKAL RQLAEAFAQG IRQRLEKAQL SFSELTWYAA PRRLAVHVSG
     LAEKQPDLEV EKRGPALQAA FDAAGAPTKA CEGFARSCGT TPDKLEKLET DKGVWLVFRS
     KQAGAPTTGL LPAIVEESLA ALPIPKRMRW GARRTEFVRP VHWIIMLFGE KVIDCEILGL
     KAGNTTLGHR FHHPAPIEIA TPANYKDALK NTGYVMADFE ERKALIRQQV EAIAKQTSGM
     AVIDEELLEE VASLNEWPTA LMGRFEDRFL EVPAEALIST MKGNQKYFHV VDAEGRMLPY
     FITVANIESK DPQQVIDGNE RVIRPRLSDA AFFFSTDKKR TLASRLEDLK PIVFQQQLGT
     VYDKAIRVGK LAAKIAAKID SNPEWAQRAG ELSKTDLATE MVMEFPELQG TMGRYYAAID
     SEPEEVSMAQ EEQYLPRFAG DQLPTTLTGC AVSLADKLDT IVGIFGINQP PTGAKDPFGL
     RRAALGVLRI LVEKKLDLDL AECVQWAQEL HGDLPAENLE TAVVDYMLDR FRAWYEEAGV
     PTEVFLSVLA RRPTKPVEFD QRVLAVAEFL KLDAAQALAA ANKRVSNILS KEQVDISAES
     ANPELFTEEA EKNLFRALQE KSESARPYIE QRNFTQALQQ LAELKDVIDL FFDKVMVMVD
     DPAIRSNRMT LLASLRHVFL QVADISLLQN KA