ID   SYGB_HALOH              Reviewed;         686 AA.
AC   B8CXH5;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=Hore_12440;
OS   Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562).
OC   Bacteria; Firmicutes; Clostridia; Halanaerobiales; Halanaerobiaceae;
OC   Halothermothrix.
OX   NCBI_TaxID=373903;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=H 168 / OCM 544 / DSM 9562;
RX   PubMed=19145256; DOI=10.1371/journal.pone.0004192;
RA   Mavromatis K., Ivanova N., Anderson I., Lykidis A., Hooper S.D., Sun H.,
RA   Kunin V., Lapidus A., Hugenholtz P., Patel B., Kyrpides N.C.;
RT   "Genome analysis of the anaerobic thermohalophilic bacterium
RT   Halothermothrix orenii.";
RL   PLoS ONE 4:E4192-E4192(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP001098; ACL69994.1; -; Genomic_DNA.
DR   RefSeq; WP_012636178.1; NC_011899.1.
DR   AlphaFoldDB; B8CXH5; -.
DR   SMR; B8CXH5; -.
DR   STRING; 373903.Hore_12440; -.
DR   PRIDE; B8CXH5; -.
DR   EnsemblBacteria; ACL69994; ACL69994; Hore_12440.
DR   KEGG; hor:Hore_12440; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_9; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000000719; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..686
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000197203"
SQ   SEQUENCE   686 AA;  78940 MW;  4DA6D6BC87E9A37D CRC64;
     MARDLLFEIG TEEMPAGLIG KIRSDLKDLA INTLEDKRLD FKECKVFSTP RRLVLFVKEL
     AEKQEEKREV VKGPARSIAF EDDGTPTRAA KGFSRAQGVE VDDLIIKDDY VYVEKIEQGQ
     DTAGLLKDIL PGLINKLPLP RSMRWADYNF KFIRPIRWLL ALFGEEIISF SMAGVQSGAY
     TRGHRFLVKD RLEVKDPDHY FDVMEKGYII VDHNKRRELI LKQIREIEKE IGKVMVEDDL
     LTEVVDLVEY PTAFYGKFDR SYLELPDDVL ITSMAEHQRY FPVIDEDGSL SPYFVGVRDG
     IEDYIEEVRY GNEMVLRARL ADARFFFEED LKVSIEERQK ELEEIVFQED LGSMMDKVKR
     LKQLVIQIGK SLNLKESQLQ SLIRAAELSK NDLVTEMVNE FTKLQGVMGR EYALINGEDE
     EVATAIYEQY LPRYSGDRLP QTLYGRILSI ADKIDNITSH FSLGMIPSGS QDPFALRRQA
     NGIVNIIIDA QLPLKLSSLL NWSIEVLEPG DKDFVNEEKS FLLQRLETIL DERGIRYDII
     NSVVRVGDDD PNNILSRAEA VMSLRKENPD LFVDLIQGLV RARNLASKGE GNNDINPEYF
     ECREEKELYD IYRKIKDEIQ RQFKKKNYLS GLKKLVDVKE PVDNFLDNVV VMVEDERIKN
     NRLALLQEIS NLVSGVMNIS EIALDD