ID   SYGB_HAMD5              Reviewed;         689 AA.
AC   C4K460;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 1.
DT   25-MAY-2022, entry version 61.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=HDEF_0612;
OS   Hamiltonella defensa subsp. Acyrthosiphon pisum (strain 5AT).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; aphid secondary symbionts; Candidatus Hamiltonella.
OX   NCBI_TaxID=572265;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=5AT;
RX   PubMed=19451630; DOI=10.1073/pnas.0900194106;
RA   Degnan P.H., Yu Y., Sisneros N., Wing R.A., Moran N.A.;
RT   "Hamiltonella defensa, genome evolution of protective bacterial
RT   endosymbiont from pathogenic ancestors.";
RL   Proc. Natl. Acad. Sci. U.S.A. 106:9063-9068(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP001277; ACQ67353.1; -; Genomic_DNA.
DR   RefSeq; WP_015873177.1; NC_012751.1.
DR   AlphaFoldDB; C4K460; -.
DR   SMR; C4K460; -.
DR   STRING; 572265.HDEF_0612; -.
DR   EnsemblBacteria; ACQ67353; ACQ67353; HDEF_0612.
DR   GeneID; 66260483; -.
DR   KEGG; hde:HDEF_0612; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_6; -.
DR   OMA; LPIPKRM; -.
DR   Proteomes; UP000002334; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..689
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000204606"
SQ   SEQUENCE   689 AA;  77624 MW;  E85132C5172DDD03 CRC64;
     MTHQTFLVEI GTEELPPKSL RSLVESFAFY LTQELNKAHL DHGEVTWFAT PRRLAVKVAC
     LSTIQKDQKI EKRGPAIAQA YDVDGNPTKA ATAWARSCGI SLDQAKSLVT DKGEWLLYSW
     VLPGKSASSL LAEKVKIALS QLPISKLMSW GIHEAKFVRP VHTVTLLLGD ELISGIIFGV
     NSNRKILGHR FMGEPSFIIE HADQYPQILL EKGKVMADFF LRKTQIKTDI EKAAEKIGAI
     ADISDNLLEE VTSLVEWPVV HTAQFEKKFL EVPSEALVHT MKNDQKYFPV YNKSGQLMPY
     FIFVANILSQ DPPQLIFGNE KVIRPRFADA QFFFETDLKQ SLEERLPSLK TILFQKELGT
     LYEKVQRVQA LSGWIASQIG ANVEYSIKAG LLSKSDLMTN MVCEFPETQG IMGMHYARYH
     HEPDEVARAI YEQYQPRFSG DNLPSTLVAC SVAIADKMDT LTGIFGINQL PKGDKDPFGL
     RRAALGVLRI IVEKNLPLDL QTLISEAVRL YGNKLKNSNL IDQIIEFMLG RFRSWYQEAG
     HGIDSIQAVL ARRPTKPADF NARIQAVTYF RTMNEARALC ASNKRVSNIL SQSLDIPKNS
     IDTGLLKEPA EIELAQNILE LEKKLAPFFV AGLYKDALLE LVALREPLDI FFKQVMVMVP
     DQDLRLNRLA LLNKLRALFL RVADISFLQ