ID   SYGB_HYPNA              Reviewed;         706 AA.
AC   Q0C4C9;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   25-MAY-2022, entry version 113.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=HNE_0685;
OS   Hyphomonas neptunium (strain ATCC 15444).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomonadales;
OC   Hyphomonadaceae; Hyphomonas.
OX   NCBI_TaxID=228405;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15444;
RX   PubMed=16980487; DOI=10.1128/jb.00111-06;
RA   Badger J.H., Hoover T.R., Brun Y.V., Weiner R.M., Laub M.T., Alexandre G.,
RA   Mrazek J., Ren Q., Paulsen I.T., Nelson K.E., Khouri H.M., Radune D.,
RA   Sosa J., Dodson R.J., Sullivan S.A., Rosovitz M.J., Madupu R.,
RA   Brinkac L.M., Durkin A.S., Daugherty S.C., Kothari S.P., Giglio M.G.,
RA   Zhou L., Haft D.H., Selengut J.D., Davidsen T.M., Yang Q., Zafar N.,
RA   Ward N.L.;
RT   "Comparative genomic evidence for a close relationship between the
RT   dimorphic prosthecate bacteria Hyphomonas neptunium and Caulobacter
RT   crescentus.";
RL   J. Bacteriol. 188:6841-6850(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000158; ABI76167.1; -; Genomic_DNA.
DR   RefSeq; WP_011645714.1; NC_008358.1.
DR   AlphaFoldDB; Q0C4C9; -.
DR   SMR; Q0C4C9; -.
DR   STRING; 228405.HNE_0685; -.
DR   EnsemblBacteria; ABI76167; ABI76167; HNE_0685.
DR   KEGG; hne:HNE_0685; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_1_5; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000001959; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..706
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101286"
SQ   SEQUENCE   706 AA;  75467 MW;  8483D903EC785BC5 CRC64;
     MADLLIELFS EEIPARMQAK AEADLLAALT GKLKEAGLAW KTAFAVSGPR RLTAVVEGLD
     ARSADVREEK KGPKVGAPEQ AIAGFLRGAG LTDISEASVV SDPKKGDFYV AYSTVPGRDA
     KDVIAEAVPA IIRDFHWPKS MRWGTGELRW VRPLQRIVCV LDGAVVPFEV GGITSGNETE
     GHRVHGRGPY TVTGWADYKS QLEGQGHVVL SRDDRRAAIL GGIEKACAKA GLQWIEDKGL
     LEEVTGLAEW PVVVLGQMDP DFLDLPPEVI TLSMRTHQKY FAATHAKTGK LAPNFILVAN
     IAATDGGAKI AEGNARVLSA RLSDARFFWE KDKATPLEVM GEKLKTIAFK EELGSLGDKV
     ERVAALAREL APAVGADPGL AERAARLAKA DLVSEMVGEF PELQGVMGRY YALAVGEDAR
     VADAIRDHYK PQGPSDSVPT DPVSIAVALA DKLDTLVGFW AIDEKPTGSK DPFALRRAAL
     GVVRINLENG VRLKLSNAIL KAPLAVRPVV KGGQSDSVRS AASKAIDALI DPTLLSFFAD
     RLKQVLRDQG KRHDLIDAVF ALGEDDLVLI VKRVEALAAF LATEDGATLL AGYRRAANIL
     KAEEKKGALP DGLSVDPALI AKGPAAEQAL WASLNETTAA LEAPLKTEDF AGAMTALAGL
     RAPVDAFFED VLVNDSDAKV RENRLALLIA VRGALHKVAD FSRIEG