SYGB_IDILO
ID SYGB_IDILO Reviewed; 688 AA.
AC Q5QY35;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 04-JAN-2005, sequence version 1.
DT 25-MAY-2022, entry version 117.
DE RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=IL0005;
OS Idiomarina loihiensis (strain ATCC BAA-735 / DSM 15497 / L2-TR).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC Idiomarinaceae; Idiomarina.
OX NCBI_TaxID=283942;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-735 / DSM 15497 / L2-TR;
RX PubMed=15596722; DOI=10.1073/pnas.0407638102;
RA Hou S., Saw J.H., Lee K.S., Freitas T.A., Belisle C., Kawarabayasi Y.,
RA Donachie S.P., Pikina A., Galperin M.Y., Koonin E.V., Makarova K.S.,
RA Omelchenko M.V., Sorokin A., Wolf Y.I., Li Q.X., Keum Y.S., Campbell S.,
RA Denery J., Aizawa S., Shibata S., Malahoff A., Alam M.;
RT "Genome sequence of the deep-sea gamma-proteobacterium Idiomarina
RT loihiensis reveals amino acid fermentation as a source of carbon and
RT energy.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:18036-18041(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC {ECO:0000255|HAMAP-Rule:MF_00255}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR EMBL; AE017340; AAV80849.1; -; Genomic_DNA.
DR RefSeq; WP_011233269.1; NC_006512.1.
DR AlphaFoldDB; Q5QY35; -.
DR SMR; Q5QY35; -.
DR STRING; 283942.IL0005; -.
DR EnsemblBacteria; AAV80849; AAV80849; IL0005.
DR KEGG; ilo:IL0005; -.
DR eggNOG; COG0751; Bacteria.
DR HOGENOM; CLU_007220_2_2_6; -.
DR OMA; LPIPKRM; -.
DR OrthoDB; 213210at2; -.
DR Proteomes; UP000001171; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR InterPro; IPR008909; DALR_anticod-bd.
DR InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR PANTHER; PTHR30075; PTHR30075; 1.
DR Pfam; PF05746; DALR_1; 1.
DR Pfam; PF02092; tRNA_synt_2f; 1.
DR PRINTS; PR01045; TRNASYNTHGB.
DR TIGRFAMs; TIGR00211; glyS; 1.
DR PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT CHAIN 1..688
FT /note="Glycine--tRNA ligase beta subunit"
FT /id="PRO_1000006369"
SQ SEQUENCE 688 AA; 76146 MW; 09AE8031B5117B57 CRC64;
MQKENLLIEL GTEELPPKAL KSLRDSLKSG IESGLKNAEL SFDSIEAYAT PRRLAVLVKQ
VETEQQDKEV EKRGPAINVA FDDTGKPTKA AEGWARSNGI TVEQADRLKT DKGEWLLHKA
TVKGQALSVL VQGILEAAIK ALPIPKPMRW GDSTAQFIRP VHTLTVMLGS ELIDAEILET
KSARFIQGHR FHSPQGFELD HVDNYLFKLR EAKVIANFEE RIETIQSEVS RLASELGGQV
IQDDELVEEV AALVEWPVAL TASFDQGFLA VPKEPLIVTM KDDQRYFPVE DGKGNLLPQF
IFITNIESRD PQQIIKGNEK VVRPRLADAQ FFFESDKKVT LESRVAALDS VLFQKQLGSI
GDKARRISVL AGKIANQLTA DVKASERAGL LCKADLVSDM VSEFPETQGV MGKHYALNDG
ESPVVAEAIE QHYWPRFSGD ELPLTKEACA VALADKLDTL VGIFGIGQVP KGDRDPFALR
RAALGLLRTL VERKLSLDLH ELLAASVEGF GDKLSNNTVA TDVFDFLLGR FRPWYQDQGI
TVDVIQSVLA RSPSKPTDFD QRVKAVQSFK SMDSAASLAA ANKRVGNILA KSDEEISGNV
DEALLQEESE TTLYRLVKQT EGAVKPLIAA GKYSDALTHL SELKEPVDAF FEHVMVNADD
DKVRINRLNL LFRLRQLFLE IADISLLQ