ID   SYGB_JANMA              Reviewed;         700 AA.
AC   A6SV18;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=mma_0425;
OS   Janthinobacterium sp. (strain Marseille) (Minibacterium massiliensis).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Oxalobacteraceae; Janthinobacterium.
OX   NCBI_TaxID=375286;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Marseille;
RX   PubMed=17722982; DOI=10.1371/journal.pgen.0030138;
RA   Audic S., Robert C., Campagna B., Parinello H., Claverie J.-M., Raoult D.,
RA   Drancourt M.;
RT   "Genome analysis of Minibacterium massiliensis highlights the convergent
RT   evolution of water-living bacteria.";
RL   PLoS Genet. 3:1454-1463(2007).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000269; ABR89000.1; -; Genomic_DNA.
DR   RefSeq; WP_012078290.1; NC_009659.1.
DR   AlphaFoldDB; A6SV18; -.
DR   SMR; A6SV18; -.
DR   STRING; 375286.mma_0425; -.
DR   EnsemblBacteria; ABR89000; ABR89000; mma_0425.
DR   KEGG; mms:mma_0425; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_4; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   BioCyc; JSP375286:MMA_RS02210-MON; -.
DR   Proteomes; UP000006388; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..700
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101287"
SQ   SEQUENCE   700 AA;  74908 MW;  2CD9B1B3381CE677 CRC64;
     MSTKNLLLEL FVEELPPKAL KKIGEAFAQT LQASLQTQGL LSADSVLTAF ASPRRLGVHL
     TKVLEQADDK TVMQKLMPAA VGIGADGKAT PALLKKLAAL GADESAVAGL KRESDGKAEV
     LFFNSVVTGV MLTDGLQKAL EETLGKLPIP KVMAYQLADG WETVNFVRPA HGLVALYGAD
     VLNISVLGLH AGNTTHGHRF EAGIDPVYIR DADSYAKQIA TEGAVIASFA ERRAEIVRQL
     ASAAAEAGSN LTPIEDEALL DEVTGLVERP NVLIGQFEEA FLEVPQECLI LTMKANQKYF
     PLLDSKGGLS NKFLIVSNIR PADASTVIGG NERVVRPRLA DAKFFFDQDR KKTLMSRVAG
     LDKVVYHNKL GTQGERIARV RAIAQAIAGK LGVDAQQADT AAQLAKADLL TDMVGEFPEL
     QGIMGRYYAL HDGLPAAVAD AIEDHYKPRF AGDELPRNPV GMVVALADKL ETLVGLFSIG
     QVPTGDKDPF ALRRHALGII RMLIEGKLDI GINDLITAAE KPFNGLSAEH RSALLNFIFD
     RLANALREQG YSAQEIDAVL ALQPQRLADV AERLAAVRAF AALPEAASLA AANKRVGNIL
     KKVEGTVAAQ VDTALLKEPA EIALNQTLTT VKPQAEAAFA RGDYTASLQA LAALRNPVDA
     FFDGVMVNAE DIALRNNRQG LLATLHQAMN QVADISKLAA