ID   SYGB_LACAC              Reviewed;         687 AA.
AC   Q5FJU0;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=LBA1198;
OS   Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactobacillus.
OX   NCBI_TaxID=272621;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700396 / NCK56 / N2 / NCFM;
RX   PubMed=15671160; DOI=10.1073/pnas.0409188102;
RA   Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L.,
RA   McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S.,
RA   Hamrick A., Cano R., Klaenhammer T.R.;
RT   "Complete genome sequence of the probiotic lactic acid bacterium
RT   Lactobacillus acidophilus NCFM.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000033; AAV43034.1; -; Genomic_DNA.
DR   RefSeq; WP_003547620.1; NC_006814.3.
DR   RefSeq; YP_194065.1; NC_006814.3.
DR   AlphaFoldDB; Q5FJU0; -.
DR   SMR; Q5FJU0; -.
DR   STRING; 272621.LBA1198; -.
DR   PRIDE; Q5FJU0; -.
DR   EnsemblBacteria; AAV43034; AAV43034; LBA1198.
DR   GeneID; 56942792; -.
DR   KEGG; lac:LBA1198; -.
DR   PATRIC; fig|272621.13.peg.1136; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_9; -.
DR   OMA; LPIPKRM; -.
DR   BioCyc; LACI272621:G1G49-1185-MON; -.
DR   Proteomes; UP000006381; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..687
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000006371"
SQ   SEQUENCE   687 AA;  78623 MW;  AF961A2B4D5728AD CRC64;
     MAKDYLFEIG TEEMPAHVVP RSVSQLADRT RKFLKENGLK FKDIKTFSTP RRLTILVEDL
     AEKQDDIDEV KKGPAKKIAQ DADGNWTKAA QGFARGQGMT TDDIYFEELK GTEYAYVHVQ
     KEGKKASDIL LGMSEIIKAM TFPTKMRWDS NDFEFVRPIH WLVSLFGNEV IPVKILDITA
     GRKTEGHRFL GDSVVLANAD DYEDALKDQY VIANAEERKD MIVNQMDELV KENHWQVKPD
     RDLLEEVTYL VEYPTVFAGS FDEKYLNIPD EVLITSMKDN QRYFEVYDEN GKLINHFIAV
     RNGNKDYLDN VISGNEKVLV ARLDDAQFFY DEDRKYPLSH FVDRLKDVSF HDKIGSMAEK
     VQRVRMIGDY LAKRWNLPEN VVKDFDRASE LYKFDLVTQM VGEFAELQGV MGMHYARLAG
     EDEEVSVAIK EHYMPATAEG PLPETTVGSL LSIADKIDTI ITFFGAGMIP TSSNDPYALR
     RYAYGIVRIL LNEKWSLPFN EVLPEIISLL NGVTPARLPK SDVDQEIADF IRDRVKQYLQ
     KNKFKYDIID AVLASSQQDP SQILAAANVL QLHHDDEEFK PVVESLTRID NILKKAKFKG
     NVEVDESLFE DNSEKELYVG VQNLQEIESL ADLYQGFVQL QPVIDQYFDV NMIMDKNEKV
     KNNRLAQLYA VSELADRLGN LSKLVIK