ID   SYGB_LACDB              Reviewed;         688 AA.
AC   Q04A23;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   14-NOV-2006, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=LBUL_1166;
OS   Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC BAA-365 / Lb-18).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactobacillus.
OX   NCBI_TaxID=321956;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-365 / Lb-18;
RX   PubMed=17030793; DOI=10.1073/pnas.0607117103;
RA   Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V.,
RA   Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V.,
RA   Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M.,
RA   Hawkins T., Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A.,
RA   Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W.,
RA   Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y.,
RA   Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R.,
RA   O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T.,
RA   Richardson P., Kozyavkin S., Weimer B.C., Mills D.A.;
RT   "Comparative genomics of the lactic acid bacteria.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000412; ABJ58699.1; -; Genomic_DNA.
DR   RefSeq; WP_011678360.1; NC_008529.1.
DR   AlphaFoldDB; Q04A23; -.
DR   SMR; Q04A23; -.
DR   KEGG; lbu:LBUL_1166; -.
DR   HOGENOM; CLU_007220_2_2_9; -.
DR   OMA; LPIPKRM; -.
DR   BioCyc; LDEL321956:LBUL_RS05455-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..688
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000006372"
SQ   SEQUENCE   688 AA;  77405 MW;  695FE2C00B932395 CRC64;
     MTKDFLFEIG VEEMPAHVVS KSVKQLADRT GKFLKENGLG FKEIKTYSTP RRLTVLVKEL
     DEKQADVDEV KKGPAKKIAQ DADGNWTKAA VGFARGQGMS ADDIYFEELK GVEYAYVHVQ
     KAGKEAKEIL LGLDEVIKAM TFPTKMRWDS QDFEFVRPIH WLVALYGSEV VPVEFLDITA
     GRKTAGHRFL GDSVVLANAD DYVEALRDQY VIVDADERKA MIVSQINDLV NSHNWQIKLD
     ASLLEEVNNL VEYPTVFAGS FDQKYLEIPD EVLITSMKDN QRYFEVYDQE GKLINCFISV
     RNGNKDHLEN VISGNEKVLV ARLDDAVFFY DEDRKYPISH FVDRMDSVSF HDKIGSMAEK
     MTRVRIIGDY LAKRFGLSAE EVADFDRVSG LYKFDLVTQM VGEFAELQGV MGMHYACLAG
     ENENVSVAIK EHYMPTTAEG DLPKTKVGAL LSVADKLDTI IAFFGAGMIP SSSNDPYALR
     RYAYGIVRIL LNEDWSLPFD QVVPEIVELL SGKTPAKLPQ GAEEDKQLAD FIRDRIKQFL
     QKNNYQYDVI DAVLASSEQD PSQILAAAKV LQQHHDDEAF KPVVESLTRI ANILKKAKFS
     QASPVDPSLF EDRSEQDLYD GVEALANVKD HAELYEAFVA LQGVIDRYFD VNMIMAKDEA
     VKNNRLSQLA AVNDLAKRLG DLSKLVIK