ID   SYGB_LACJO              Reviewed;         690 AA.
AC   Q74IZ2;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 122.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=LJ_1319;
OS   Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactobacillus.
OX   NCBI_TaxID=257314;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CNCM I-1225 / La1 / NCC 533;
RX   PubMed=14983040; DOI=10.1073/pnas.0307327101;
RA   Pridmore R.D., Berger B., Desiere F., Vilanova D., Barretto C.,
RA   Pittet A.-C., Zwahlen M.-C., Rouvet M., Altermann E., Barrangou R.,
RA   Mollet B., Mercenier A., Klaenhammer T., Arigoni F., Schell M.A.;
RT   "The genome sequence of the probiotic intestinal bacterium Lactobacillus
RT   johnsonii NCC 533.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:2512-2517(2004).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; AE017198; AAS09139.1; -; Genomic_DNA.
DR   RefSeq; WP_004897198.1; NC_005362.1.
DR   AlphaFoldDB; Q74IZ2; -.
DR   SMR; Q74IZ2; -.
DR   STRING; 257314.LJ_1319; -.
DR   PRIDE; Q74IZ2; -.
DR   EnsemblBacteria; AAS09139; AAS09139; LJ_1319.
DR   KEGG; ljo:LJ_1319; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_9; -.
DR   OMA; LPIPKRM; -.
DR   Proteomes; UP000000581; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..690
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000006374"
SQ   SEQUENCE   690 AA;  78024 MW;  ECEA0F7EA388CA6A CRC64;
     MTKDYLFEIG TEEMPAHVVS KSVKQLADRT KKYLKENGLS FKDIKTYSTP RRLTILVEDL
     AEKQEDIDEV KKGPAKKIAQ DKDGNWTKAA QGFVRGQGMT TDDIYFEELK GTEYAYVHVQ
     KEGKKASDIL MGMSDIVKAM TFPTKMRWGS YDFEFVRPIH WMVSLLGSEV VPVKLLDVVA
     GRKTQGHRFL GDSVVLANAD DYEEALKSQY VIANADERKG MILNQIQELV AQHNWKVNID
     KGLLEEVTNL VEYPTVFAGS FDEKYLNIPD EVLITSMKDN QRYFEVYDEN GKLINHFISV
     RNGNSEYLDN VIAGNEKVLV ARLDDAQFFY DEDKKYPLAH FVDKLKNVSF HDKIGSVAEH
     MARVQIIGDY LGKKFNVSDT EMKDFDRVSD IYKFDLVTSM VGEFAELQGV MGMHYARLIG
     ENENVSVAIK ESYMPTSAEG DLPSTTVGSL LSIADKLDTI ISFFGAGMIP SSSNDPYALR
     RNAYGIVRIL LNEGWSLPIK DVLPELIQLL SGKTAAKLPK DAEAENEIAD FIRDRVKQQL
     QVEKYDYDVI DAVLASSQQD PIQILAAAKT LQMHHDDADF KPVVESLTRI TNILKKAKYR
     NANDIDESLF QDVSEEELYA GVNALEENTD LSIADLYKGF VELQPVINNY FESNMILDKD
     EKVKNNRLAQ LLKVNNLADR MGDLSKLVIK