ID   SYGB_LACLM              Reviewed;         673 AA.
AC   A2RL88;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   06-MAR-2007, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=llmg_1477;
OS   Lactococcus lactis subsp. cremoris (strain MG1363).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus; Lactococcus cremoris subsp. cremoris.
OX   NCBI_TaxID=416870;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MG1363;
RX   PubMed=17307855; DOI=10.1128/jb.01768-06;
RA   Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C.,
RA   Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P.,
RA   van Sinderen D., Kok J.;
RT   "The complete genome sequence of the lactic acid bacterial paradigm
RT   Lactococcus lactis subsp. cremoris MG1363.";
RL   J. Bacteriol. 189:3256-3270(2007).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; AM406671; CAL98055.1; -; Genomic_DNA.
DR   RefSeq; WP_011835326.1; NZ_WJVF01000002.1.
DR   AlphaFoldDB; A2RL88; -.
DR   SMR; A2RL88; -.
DR   STRING; 416870.llmg_1477; -.
DR   EnsemblBacteria; CAL98055; CAL98055; llmg_1477.
DR   KEGG; llm:llmg_1477; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_9; -.
DR   OMA; LPIPKRM; -.
DR   PhylomeDB; A2RL88; -.
DR   BioCyc; LLAC416870:LLMG_RS07450-MON; -.
DR   Proteomes; UP000000364; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..673
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101293"
SQ   SEQUENCE   673 AA;  75642 MW;  F85E2D9218E5DE13 CRC64;
     MTNYLLEIGL EEIPAHLVTP SINQLAERME AFLNENRLKF DKIIKFSTPR RLALIVEELS
     DSSEAIDEEV KGPSAKIAKD AEGNWSKAIQ GFSRGQGATP DDLILKGDYY YAKKHVDGVK
     SEEILSKVGD EVIAKMTFST YMKWGNNDFL FVRPIQWIVS LLEDEIVAFD LLDVTANRFS
     RGHRFLANVE IELKNANDYA SKMPENFVLV DAEHRKAEIS AQILALASEN NWQVTLHKDL
     LEEVNNIVEY PTAFVGSFDP KYLSVPAEVL VTSMRDNQRY FEVYNQEGQL APNFISVRNG
     NAEHIENVVL GNEKVLFARL EDAEFFWKED QKLKIEDLVA KLAKVTFHAK IGSITEHMAR
     TKLIAAKLAD IAGLTDEEKV DVARSAEIYK FDLLTGMVGE FDELQGVMGE KYALLAGENA
     NVAAAIREHY MPTSADGQLP ETKVGSVLAA ADKIDSVLSF FNVGLIPSGS NDPYALRRAV
     QGLIRIIEKM NWHFDLSLFI DQFEGQNHAE ILEFVKARVQ KLLLEKLDRY DIVEAAINSS
     NFDITNMMES AFVIDGHKLH EPFKPAIENV SRSINLVKKA ADIAEINPAL FEEDTEQALY
     DAVISLQNQW TYKPCEEKFR AIVHMLAPAI EAFFDNVMVM AEDLAVRDNR IALLSEVVAL
     TSVMADFSLI NTK