ID   SYGB_LACLS              Reviewed;         673 AA.
AC   Q02Z93;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   14-NOV-2006, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=LACR_1197;
OS   Lactococcus lactis subsp. cremoris (strain SK11).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus; Lactococcus cremoris subsp. cremoris.
OX   NCBI_TaxID=272622;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SK11;
RX   PubMed=17030793; DOI=10.1073/pnas.0607117103;
RA   Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V.,
RA   Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V.,
RA   Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M.,
RA   Hawkins T., Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A.,
RA   Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W.,
RA   Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y.,
RA   Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R.,
RA   O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T.,
RA   Richardson P., Kozyavkin S., Weimer B.C., Mills D.A.;
RT   "Comparative genomics of the lactic acid bacteria.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP000425; ABJ72729.1; -; Genomic_DNA.
DR   RefSeq; WP_011676102.1; NC_008527.1.
DR   AlphaFoldDB; Q02Z93; -.
DR   SMR; Q02Z93; -.
DR   EnsemblBacteria; ABJ72729; ABJ72729; LACR_1197.
DR   KEGG; llc:LACR_1197; -.
DR   HOGENOM; CLU_007220_2_2_9; -.
DR   OMA; LPIPKRM; -.
DR   Proteomes; UP000000240; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..673
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101294"
SQ   SEQUENCE   673 AA;  75476 MW;  C17B0FDA100CCE2D CRC64;
     MTNYLLEIGL EEIPAHLVTP SINQLAERME AFLNENRLKF DKIIKFSTPR RLALIVEGLS
     ESSEAIDEEV KGPSAKIAKD AEGNWSKAIQ GFSRGQGATP DDLILKGDYY YAKKHVDGVK
     AEEILSKVGD EVIAKMTFST YMKWGNNDFL FVRPIQWIVS LLEDEIVAFD LLDVTANRFS
     RGHRFLANVE IELKNANDYA SKMPENFVLV DAEHRKAEIS AQILALASEN NWQVTLHKDL
     LEEVNNIVEY PTAFVGSFDP KYLSVPAEVL VTSMRDNQRY FEVYNQEGQL APNFISVRNG
     NAEHIENVVL GNEKVLVARL EDAEFFWKED QKLKIEDLVA KLGKVTFHAK IGSITEHMAR
     TKLIAAKLAD IAGLTDEEKV DVARSAEIYK FDLLTGMVGE FDELQGVMGE KYALLAGENA
     NVAAAIREHY MPTSADGQLP ETKVGSVLAA ADKIDSVLSF FNVGLIPSGS NDPYALRRAV
     QGLIRIIEKM NWHFDLSLFI DQFEGQNHAE ILEFVKARVQ KLLLEKLDRY DIVEAAINSS
     NFDITNMMES AFVIDGHKLH EPFKPAIENV SRSINLVKKA ADIAEINPAL FEEDTEQALY
     DAVISLQNQW TYKPCEEKFR AIVHTLAPAI EAFFDNVMVM AEDLAVRDNR IALLSEVVAL
     TSVMADFSLI NTK