ID   SYGB_LACPL              Reviewed;         694 AA.
AC   Q88VS3; F9UPS9;
DT   31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=lp_1964;
OS   Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
OS   (Lactobacillus plantarum).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactiplantibacillus.
OX   NCBI_TaxID=220668;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX   PubMed=12566566; DOI=10.1073/pnas.0337704100;
RA   Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P.,
RA   Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J.,
RA   Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M.,
RA   Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M.,
RA   Siezen R.J.;
RT   "Complete genome sequence of Lactobacillus plantarum WCFS1.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX   PubMed=22156394; DOI=10.1128/jb.06275-11;
RA   Siezen R.J., Francke C., Renckens B., Boekhorst J., Wels M.,
RA   Kleerebezem M., van Hijum S.A.;
RT   "Complete resequencing and reannotation of the Lactobacillus plantarum
RT   WCFS1 genome.";
RL   J. Bacteriol. 194:195-196(2012).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; AL935263; CCC79218.1; -; Genomic_DNA.
DR   RefSeq; WP_011101608.1; NC_004567.2.
DR   RefSeq; YP_004889732.1; NC_004567.2.
DR   AlphaFoldDB; Q88VS3; -.
DR   SMR; Q88VS3; -.
DR   STRING; 220668.lp_1964; -.
DR   EnsemblBacteria; CCC79218; CCC79218; lp_1964.
DR   KEGG; lpl:lp_1964; -.
DR   PATRIC; fig|220668.9.peg.1658; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_9; -.
DR   OMA; LPIPKRM; -.
DR   PhylomeDB; Q88VS3; -.
DR   BioCyc; LPLA220668:G1GW0-1682-MON; -.
DR   Proteomes; UP000000432; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..694
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_0000072909"
SQ   SEQUENCE   694 AA;  78532 MW;  37752F3F0DA95B1E CRC64;
     MAKTYLLEIG LEEMPAHVVT PSVLQLKERM IKFLKDARLD FEDVKTFSTP RRLTVQVLGL
     ADKQADVKKE VRGPAKKIAQ DADGNWTKAA IGFSKGQGAS TDDIVFKDVK GTPYVFVQTF
     TAGKTAAEVL TGGIKDVITK MNFPTMMKWS TYSFKYIRPI RWIVSLLDDE VVPVQILDVA
     AGRVSRGHRF LGHDVEIATA ADYEADLASV QVVADAAKRK ATIREQIAAL ANERDWQIKV
     NEDLLEEVNN LVEYPTAFAG DFDTKYLTIP DEVLITSMRD HQRFFYVTDA EDNLLPHFVS
     VRNGNTDHLE NVALGNQKVL TARLEDAAFF YHEDQQHSIQ EYVERLKKVS FHDKIGTMYE
     KMQRVMIISD FLADRFGLTE TEKNQLHRAA QIYKFDLVTG MVGEFPELQG VMGDKYAVLK
     GEDPAVGQAI REHYMPISAD GDLPKSKVGA VLAIADKVDS ITSFFAVGLT PSGSNDPFAL
     RRQAFGIVRI VREQGWDFPI RQLEADIQKE LVAHDATYNL DFEKQTAPVA DFLTDRVKQW
     FNNRKIRYDI VDTVIKGSRQ DIREMFKAAD VLNAHQDDPQ FKDTIEAFTR LLRITAKAKL
     AADDLTVDPS LFENEAEQHL YDAVLELQKQ FTPAMSMEDR FKALAALRPL IVDYFEQTMV
     MSKDEKVRDN HLKQLLTIAQ MINVMGDLNQ LIVK