ID   SYGB_LATSS              Reviewed;         693 AA.
AC   Q38X99;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=LCA_0881;
OS   Latilactobacillus sakei subsp. sakei (strain 23K) (Lactobacillus sakei
OS   subsp. sakei).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Latilactobacillus.
OX   NCBI_TaxID=314315;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=23K;
RX   PubMed=16273110; DOI=10.1038/nbt1160;
RA   Chaillou S., Champomier-Verges M.-C., Cornet M., Crutz-Le Coq A.-M.,
RA   Dudez A.-M., Martin V., Beaufils S., Darbon-Rongere E., Bossy R., Loux V.,
RA   Zagorec M.;
RT   "The complete genome sequence of the meat-borne lactic acid bacterium
RT   Lactobacillus sakei 23K.";
RL   Nat. Biotechnol. 23:1527-1533(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CR936503; CAI55182.1; -; Genomic_DNA.
DR   RefSeq; WP_011374583.1; NC_007576.1.
DR   AlphaFoldDB; Q38X99; -.
DR   SMR; Q38X99; -.
DR   STRING; 314315.LCA_0881; -.
DR   EnsemblBacteria; CAI55182; CAI55182; LCA_0881.
DR   KEGG; lsa:LCA_0881; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_9; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   BioCyc; LSAK314315:LCA_RS04425-MON; -.
DR   Proteomes; UP000002707; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..693
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000006376"
SQ   SEQUENCE   693 AA;  77774 MW;  2BC39C4C856AB3E5 CRC64;
     MTKPFLLEIG LEEMPAHVVM PSIQQLEKRV RDFLEEQHLE FGQLKTYATP RRLAILIEDL
     ADKQADIHEE AKGPAKKIAL DAEGNWSKAA QGFVRGQGLT TDDITFKELK GVEYVYVEKS
     IIGKPVEAIL PELKAVIMAM TFPTRMHWAD YDFEYIRPIH WIIALLDDEV IDFSILNVTT
     GRTTQGHRFL GQEVTLAKAT DYEAALEAQF VIADEAKRQN LIQTQIEKMA QENDWQVDLD
     ANLLEEVTNL VEWPTAFYGQ FDAKYLEIPE EVLITSMKDN QRYFYARSQA GDLLPVFIGV
     RNGNDAFMEN VIAGNEKVLT ARLEDADFFY HEDQKVTIAE NVAKLQNVSF HDKIGSLAEK
     MTRVGLIAQF LGQKFGLSEA ELVDLQRASD IYKFDLVTGM VGEFAELQGV MGEKYALLQG
     ETPAVATAVR EHYMPISAEG ALPATKVGAV LAIADKFDSM ADFFAVGMIP SGSNDPYALR
     RQAYGIVRIL ENQDWHFPMA DFQAAMSHIV KQQADFDLDW SANAAAISDF LTDRVRQRFN
     GMRPKVRRDI VDAVLKNQGQ DPVVMFEAAK VLSAHQDDAD FKATIEAITR VLRLAEKVDF
     ETTDLTVDPA LFENDAEKAL YTAVEDLRGA TATNTLAENY AALQTLRPLI EAYFDATMVM
     AEDEAVRRNR LTQLMIIAQI ALSLGDLNEL IVK