ID   SYGB_LEPCP              Reviewed;         713 AA.
AC   B1Y7P2;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            EC=6.1.1.14 {ECO:0000255|HAMAP-Rule:MF_00255};
DE   AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000255|HAMAP-Rule:MF_00255};
DE            Short=GlyRS {ECO:0000255|HAMAP-Rule:MF_00255};
GN   Name=glyS {ECO:0000255|HAMAP-Rule:MF_00255}; OrderedLocusNames=Lcho_0215;
OS   Leptothrix cholodnii (strain ATCC 51168 / LMG 8142 / SP-6) (Leptothrix
OS   discophora (strain SP-6)).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Leptothrix.
OX   NCBI_TaxID=395495;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51168 / LMG 8142 / SP-6;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C.,
RA   Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.,
RA   Kyrpides N., Lykidis A., Emerson D., Richardson P.;
RT   "Complete sequence of Leptothrix cholodnii SP-6.";
RL   Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC         tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC         COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC         EC=6.1.1.14; Evidence={ECO:0000255|HAMAP-Rule:MF_00255};
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00255}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00255}.
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DR   EMBL; CP001013; ACB32490.1; -; Genomic_DNA.
DR   RefSeq; WP_012345252.1; NC_010524.1.
DR   AlphaFoldDB; B1Y7P2; -.
DR   SMR; B1Y7P2; -.
DR   STRING; 395495.Lcho_0215; -.
DR   PRIDE; B1Y7P2; -.
DR   EnsemblBacteria; ACB32490; ACB32490; Lcho_0215.
DR   KEGG; lch:Lcho_0215; -.
DR   eggNOG; COG0751; Bacteria.
DR   HOGENOM; CLU_007220_2_2_4; -.
DR   OMA; LPIPKRM; -.
DR   OrthoDB; 213210at2; -.
DR   Proteomes; UP000001693; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR   InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR   PANTHER; PTHR30075; PTHR30075; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF02092; tRNA_synt_2f; 1.
DR   PRINTS; PR01045; TRNASYNTHGB.
DR   TIGRFAMs; TIGR00211; glyS; 1.
DR   PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..713
FT                   /note="Glycine--tRNA ligase beta subunit"
FT                   /id="PRO_1000101296"
SQ   SEQUENCE   713 AA;  75682 MW;  DC4330E926B35CF6 CRC64;
     MSHPNLLVEL FVEELPPKAL KKLGESFAGT LAASLKAGGL AGADAVVTPF ASPRRLAVHV
     TGVAAKAADK SVLHKLMPVA VALDAAGNAT PALLKKLAAL GADASVVPSL KRQPDGKAEA
     LFLDSQVAGA TLAEGLQKAL DDALAKLPIP KVMGYQLADG WTSVNFVRPA HGLVALHGDD
     VVPVATLGLV AGRSTHGHRF EAAVPTVELR HADSYAEQLE TEGAVIAGFE ARRAEIVRQL
     DAAAAAQGLK PIDDDALLDE VTALVERPNV LVCQFEPEFL AVPQECLILT MKANQKYFPL
     LDAAGKLTHK FLIVSNIRPD DASAVIGGNE RVVRPRLADA KFFFDQDRKK TLESRVPGLA
     RVVYHGKLGT QGERAERVRA IGHAIVNQLR MATIPYTVDA QDEFAVLDSK VQQAALLAKT
     DLLTDMVGEF PELQGIMGGY YARHEGLRDG VAIAIEDHYK PRFAGDALPR NHTGTVLALA
     DKLETLVGLF GIGQLPTGDR DPFALRRHAL GVIRILVEKN LPLDLPALLN GAVPAFGELI
     EDPRLALFDF MRDRLAVNLR DQGYSAQEVD AVLALEPARL GDVPKRLAAV RAFAALPEAA
     ALAAANKRIG NILKKAEGTV EARIDAALLA EPAEQQLAAA LAQVQPGADA LFAQGEYAAS
     LQALAALKAP VDAFFDDVMV NAEDPALRAN RLGLLATLHG AMNRVAELAR LAA